V-myc immortalizes human neural stem cells in the absence of pluripotency-associated traits

PloS One
María José Pino-BarrioAlberto Martínez-Serrano

Abstract

A better understanding of the molecular mechanisms governing stem cell self-renewal will foster the use of different types of stem cells in disease modeling and cell therapy strategies. Immortalization, understood as the capacity for indefinite expansion, is needed for the generation of any cell line. In the case of v-myc immortalized multipotent human Neural Stem Cells (hNSCs), we hypothesized that v-myc immortalization could induce a more de-differentiated state in v-myc hNSC lines. To test this, we investigated the expression of surface, biochemical and genetic markers of stemness and pluripotency in v-myc immortalized and control hNSCs (primary precursors, that is, neurospheres) and compared these two cell types to human Embryonic Stem Cells (hESCs) and fibroblasts. Using a Hierarchical Clustering method and a Principal Component Analysis (PCA), the v-myc hNSCs associated with their counterparts hNSCs (in the absence of v-myc) and displayed a differential expression pattern when compared to hESCs. Moreover, the expression analysis of pluripotency markers suggested no evidence supporting a reprogramming-like process despite the increment in telomerase expression. In conclusion, v-myc expression in hNSC lines ensures self-ren...Continue Reading

References

Dec 1, 1996·Current Biology : CB·M Zörnig, G I Evan
Nov 19, 1997·Trends in Neurosciences·A Martínez-Serrano, A Björklund
Nov 6, 1998·Science·J A ThomsonJ M Jones
Apr 11, 2001·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·A VillaA Martínez-Serrano
Mar 17, 2004·Experimental Cell Research·Ana VillaAlberto Martinez-Serrano
May 26, 2006·The EMBO Journal·Paul S KnoepflerRobert N Eisenman
Dec 13, 2006·Experimental Cell Research·E CacciZ Kokaia
Jun 5, 2007·Cancer Research·Paul S Knoepfler
Jun 19, 2007·Nature Biotechnology·UNKNOWN International Stem Cell InitiativeWeidong Zhang
Aug 29, 2007·Nature Biotechnology·Alexander MeissnerRudolf Jaenisch
Dec 7, 2007·Nature Biotechnology·Masato NakagawaShinya Yamanaka
Mar 22, 2008·Differentiation; Research in Biological Diversity·Gabriela Durcova-Hills
Mar 29, 2008·Cell Stem Cell·Marius WernigRudolf Jaenisch
Mar 29, 2008·Cell Stem Cell·Paul S Knoepfler
Mar 29, 2008·Cell Stem Cell·Matthias StadtfeldKonrad Hochedlinger
May 30, 2008·Nature·Tarjei S MikkelsenAlexander Meissner
Nov 26, 2008·Nature Reviews. Cancer·Natalie Meyer, Linda Z Penn
Dec 2, 2008·Cell Stem Cell·Nimet Maherali, Konrad Hochedlinger
Feb 11, 2009·Cell·Jeong Beom KimHans R Schöler
Mar 31, 2009·Experimental Cell Research·Ana VillaAlberto Martínez-Serrano
Sep 8, 2009·Nature Medicine·Rahul Jandial, Evan Y Snyder
Sep 24, 2009·Cancer Research·Natalia V Varlakhanova, Paul S Knoepfler
Mar 20, 2010·Development, Growth & Differentiation·Kazutoshi Takahashi
Aug 26, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Veronica Ramos-MejiaPablo Menendez
Sep 2, 2010·Cell Stem Cell·Keriayn N SmithStephen Dalton
Oct 27, 2010·Stem Cell Research & Therapy·Jie NaChristian Unger
Mar 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kwang S KimSeung U Kim
Apr 12, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Pedro J RealPablo Menendez
Dec 25, 2012·Cell·Jose M PoloKonrad Hochedlinger
Nov 5, 2013·Neuron·Fred H Gage, Sally Temple

❮ Previous
Next ❯

Citations

Mar 10, 2018·Chinese Medical Journal·Shuang-Lin DengGang Zhao
Jan 28, 2021·International Journal of Molecular Sciences·Mafalda Giovanna RecciaLuca Colucci-D'Amato

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE63710

Methods Mentioned

BETA
PCA

Software Mentioned

R Program
Adobe PostScript
Adobe Photoshop
R Project for Statistical Computing
Statistica
Adobe Design

Related Concepts

Related Feeds

Cancer Epigenetics

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. Here is the latest research on cancer epigenetics.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Cell Signaling & Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. This feed covers the latest research on signaling and epigenetics in cell growth and cancer.

Cancer Epigenetics & Methyl-CpG (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. Here is the latest research on cancer epigenetics and methyl-CpG binding proteins including ZBTB38.

Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. Here is the latest research on cancer epigenetics.

Cancer Epigenetics & Metabolism (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on the relationship between cell metabolism, epigenetics and tumor differentiation.