Vacancy ion-exclusion/adsorption chromatography of aliphatic amines on a polymethacrylate-based weakly basic anion-exchange column

Journal of Chromatography. a
Masanobu MoriKazuhiko Tanaka

Abstract

Vacancy ion-exclusion/adsorption chromatography has been applied to investigate the separation behavior of five aliphatic amines (ethylamine, propylamine, butylamine, pentylamine and hexylamine) on a polymethacrylate-based weakly basic anion-exchange column (Tosoh TSKgel DEAE-5PW). This system is consisted of analytes as a mobile phase and water as an injected sample. In the vacancy ion-exclusion/adsorption chromatography, the elution order was as follows: ethylamine < propylamine < butylamine < pentylamine < hexylamine, depending on their hydrophobicity. The retention times of the amines were decreased with decreasing their concentrations in the mobile phase. The retention times and resolutions of the amines were increased by adding a basic compound (e.g., lithium hydroxide or heptylamine) and by increasing the pH of mobile phase (pH > 11). This was because the dissociations of amine samples in the mobile phase were suppressed and thus the hydrophobic adsorption effects were enhanced. The linearity of calibration graphs could be obtained from the peak areas of the amine samples injected to the 0.05, 0.5 and 5 mM of amine mobile phase at pH 11 by heptylamine. The detection limits of aliphatic amines as injected samples were aro...Continue Reading

Citations

Jan 11, 2013·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Daisuke KozakiKazuhiko Tanaka
Sep 15, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nobutake NakataniKazuhiko Tanaka
Feb 14, 2006·Journal of Chromatography. a·Masanobu MoriKazuhiko Tanaka
Oct 20, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kazuhiko Tanaka, Masanobu Mori

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