Vaccarin attenuates high glucose-induced human EA•hy926 endothelial cell injury through inhibition of Notch signaling

Molecular Medicine Reports
Yuyu QiuLiying Qiu

Abstract

Endothelial cell injury is a critical component of atherosclerosis and hypertension. Vaccarin is considered to be of potential benefit in the treatment of vascular diseases. The aim of the present study was to evaluate the possible effects of vaccarin in human EA·hy926 cells induced by high glucose, and to investigate its underlying mechanism in the prevention and treatment of high glucose‑induced injury. In the present study, EA·hy926 cells were exposed to 90, 180 and 270 mM high glucose for 24 h, and the induced cell injury was examined using a sulforhodamine B assay. Following treatment with high glucose, it was found that high glucose stimulated cell injury, resulting in reduced cell viability and migratory ability, increased lactate dehydrogenase (LDH) leakage and malondialdehyde (MDA) levels, and decreased superoxide dismutase (SOD) activity. High glucose further accelerated cell apoptosis via activating Notch1 and Hairy and enhancer of split 1. It was found that preincubation with vaccarin protected the EA·hy926 cells from high glucose‑induced cell injury, which promoted cell viability and migratory ability, inhibited the expression levels of LDH and MDA, and enhanced the activity of SOD. Cell migratory ability, LDH leak...Continue Reading

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Citations

May 28, 2019·Journal of Cellular Biochemistry·Yugang Yin, Chun Yang
Aug 31, 2016·Molecular Medicine Reports·Zhiyou CaiGuanghui Chen
Nov 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xuelin ShiLiying Qiu
Sep 20, 2019·International Journal of Molecular Sciences·Fei XuLiying Qiu
Mar 19, 2020·International Journal of Molecular Sciences·Yixiao LiuLiying Qiu
Aug 2, 2017·International Journal of Molecular Sciences·Ding-Yuan TianYun Wang
Aug 26, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hai-Jian SunLi-Ying Qiu
Apr 11, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Huxinyue DuanChunjie Wu
Jul 26, 2021·European Journal of Pharmacology·Jiang-Nan SunLi-Ying Qiu

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