Abstract
Marek's disease virus (MDV), a causative agent of Marek's disease, has evolved its virulence partly because the current control strategies fail to provide sterilizing immunity. Gallid alphaherpesvirus 3 (GaHV-3) and turkey herpesvirus have been developed as bivalent vaccines to improve upon the level of protection elicited by single formulations. Since the in vitro passage of vaccines can result in attenuation, a GaHV-3 strain 301B/1 was cloned as a bacterial artificial chromosome (BAC) by inserting the mini-F replicon into the virus genome. A fully infectious virus, v301B-BAC, was reconstituted from the 301B/1 BAC clone and had similar growth kinetics comparable to that of the parental 301B/1 virus with strong reactivity against anti-301B/1 chicken sera. Protective efficacies of v301B-BAC, parental 301B/1, and SB-1 vaccine were evaluated against a very virulent MDV Md5 challenge. Clinical signs were significantly lower in the v301B-BAC vaccinated groups (24-25 %), parental 301B/1 (29 %) compare to that of non-vaccinated control (100%) and the removal of BAC sequences from v301B-BAC genome further reduced this to 17 %. The protective indices of v301B-BACs (75-76 %) were comparable with those of both the 301B/1 and the SB-1 vacc...Continue Reading
References
Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R IsfortH J Kung
Dec 1, 1985·Poultry Science·R L WitterV Sivanandan
Aug 15, 1994·Virology·R J IsfortH J Kung
May 20, 1998·BioTechniques·U Arad
Nov 9, 2000·Journal of Virology·D SchumacherN Osterrieder
Feb 24, 2001·Current Topics in Microbiology and Immunology·Y IzumiyaT Mikami
Jul 30, 2003·Journal of Virology·Lawrence PetherbridgeVenugopal Nair
Sep 9, 2005·Avian Pathology : Journal of the W.V.P.A·I M GimenoR F Silva
Mar 11, 2006·BioTechniques·B Karsten TischerNikolaus Osterrieder
Mar 11, 2006·The Journal of General Virology·Susan J BaigentVenugopal K Nair
Jan 31, 2008·Virology Journal·Yuguang ZhaoVenugopal Nair
Jan 1, 1984·Avian Pathology : Journal of the W.V.P.A·R L Witter, L F Lee
Jan 1, 1982·Avian Pathology : Journal of the W.V.P.A·R L Witter
Jan 1, 1982·Avian Pathology : Journal of the W.V.P.A·K A SchatJ Fabricant
Feb 4, 2009·Journal of Virological Methods·Lawrence PetherbridgeVenugopal Nair
Feb 11, 2010·Research in Veterinary Science·S M SinghV K Nair
May 5, 2010·Current Protocols in Microbiology·Raquel Hernandez, Dennis T Brown
Dec 3, 2010·The Journal of General Virology·Masahiro NiikuraHans H Cheng
Feb 22, 2011·Virus Genes·Stephen J Spatz, Karel A Schat
Feb 9, 2013·Bioscience, Biotechnology, and Biochemistry·Mayank SaraswatWayne M Patrick
Aug 2, 2013·Avian Diseases·Blanca LupianiSanjay M Reddy
Jun 11, 2016·The Journal of General Virology·Maricarmen GarciaG H Schneiders
Dec 3, 2016·Avian Diseases·Karel A Schat
Feb 25, 2018·Current Opinion in Virology·Michael D BaronVenugopal Nair
Jun 7, 2018·NPJ Vaccines·Yashar SadighVenugopal Nair
Jun 30, 2019·Avian Diseases·John R DunnAngela Hartman