Vaccination Expands Antigen-Specific CD4+ Memory T Cells and Mobilizes Bystander Central Memory T Cells

PloS One
Eleonora Li CausiGianfranco Di Genova

Abstract

CD4+ T helper memory (Thmem) cells influence both natural and vaccine-boosted immunity, but mechanisms for their maintenance remain unclear. Pro-survival signals from the common gamma-chain cytokines, in particular IL-7, appear important. Previously we showed in healthy volunteers that a booster vaccination with tetanus toxoid (TT) expanded peripheral blood TT-specific Thmem cells as expected, but was accompanied by parallel increase of Thmem cells specific for two unrelated and non cross-reactive common recall antigens. Here, in a new cohort of healthy human subjects, we compare blood vaccine-specific and bystander Thmem cells in terms of differentiation stage, function, activation and proliferative status. Both responses peaked 1 week post-vaccination. Vaccine-specific cytokine-producing Thmem cells were predominantly effector memory, whereas bystander cells were mainly of central memory phenotype. Importantly, TT-specific Thmem cells were activated (CD38High HLA-DR+), cycling or recently divided (Ki-67+), and apparently vulnerable to death (IL-7RαLow and Bcl-2 Low). In contrast, bystander Thmem cells were resting (CD38Low HLA-DR- Ki-67-) with high expression of IL-7Rα and Bcl-2. These findings allow a clear distinction betwe...Continue Reading

References

Aug 1, 1994·European Journal of Immunology·V FernandezM Troye-Blomberg
Apr 1, 1996·Journal of Virology·W E DemkowiczF A Ennis
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·A T VellaP Marrack
Aug 31, 2000·Annual Review of Biochemistry·A StrasserV M Dixit
Feb 13, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S M VargaR M Welsh
Aug 20, 2003·Nature Medicine·Erika HammarlundMark K Slifka
Nov 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shane CrottyRafi Ahmed
Mar 23, 2004·Annual Review of Immunology·Federica SallustoAntonio Lanzavecchia
Jul 5, 2005·Journal of Immunological Methods·George B CohenR Paul Johnson
Sep 28, 2005·Nature Medicine·Pratip K ChattopadhyayMario Roederer
Mar 21, 2007·European Journal of Immunology·Cristina CelleraiGiuseppe Pantaleo
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sarah C BangsXiao-Ning Xu
Aug 13, 2009·European Journal of Immunology·Federica Sallusto, Antonio Lanzavecchia
Nov 3, 2009·Cytokine·Lisa Colleen Osborne, Ninan Abraham
Nov 17, 2009·Journal of Leukocyte Biology·Gema Jiménez-GómezJosé A Brieva
Jan 28, 2010·European Journal of Immunology·Gianfranco Di GenovaFreda K Stevenson
Feb 16, 2010·Nature Reviews. Immunology·Koji TokoyodaAndreas Radbruch
Mar 3, 2010·Annual Review of Immunology·Jinfang ZhuWilliam E Paul
Apr 27, 2010·Nature Immunology·Georgia Perona-WrightMarkus Mohrs
Apr 29, 2011·Current Opinion in Immunology·Justin J Taylor, Marc K Jenkins
May 13, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dietmar Herndler-BrandstetterBeatrix Grubeck-Loebenstein
Sep 9, 2011·Journal of Virology·Maya F KotturiAlessandro Sette
Oct 21, 2011·Journal of Leukocyte Biology·Dietmar Herndler-BrandstetterBeatrix Grubeck-Loebenstein
Apr 5, 2012·Proceedings of the National Academy of Sciences of the United States of America·Kenta ShinodaToshinori Nakayama
Jun 27, 2012·European Journal of Immunology·Siegfried KohlerAndreas Thiel
Jun 14, 2014·Proceedings of the National Academy of Sciences of the United States of America·Anna OkhrimenkoJun Dong

❮ Previous
Next ❯

Citations

Mar 13, 2018·The Journal of Infectious Diseases·Anthony L CunninghamUNKNOWN ZOE-50/70 Study Group
Sep 20, 2018·Journal of Cachexia, Sarcopenia and Muscle·Tobias WinklerHans-Dieter Volk
Aug 31, 2016·Nature Communications·Hannes UchtenhagenEddie A James
Jul 17, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Benjamin J MeckiffHeather M Long
Jun 6, 2021·Journal of Immunological Methods·Jessica S DuprezD Andrew James
Jul 6, 2021·Frontiers in Immunology·Anne S De GrootPriya S Kishnani

❮ Previous
Next ❯

Methods Mentioned

BETA
blood collection

Software Mentioned

GraphPad
Treestar
SPSS
FlowJo
GraphPad Prism

Related Concepts

Related Feeds

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.