Vaccination of BALB/c mice with Escherichia coli-expressed vaccinia virus proteins A27L, B5R, and D8L protects mice from lethal vaccinia virus challenge

Journal of Virology
Aklile BerhanuDennis E Hruby

Abstract

The potential threat of smallpox use in a bioterrorist attack has heightened the need to develop an effective smallpox vaccine for immunization of the general public. Vaccination with the current smallpox vaccine, Dryvax, produces protective immunity but may result in adverse reactions for some vaccinees. A subunit vaccine composed of protective vaccinia virus proteins should avoid the complications arising from live-virus vaccination and thus provide a safer alternative smallpox vaccine. In this study, we assessed the protective efficacy and immunogenicity of a multisubunit vaccine composed of the A27L and D8L proteins from the intracellular mature virus (IMV) form and the B5R protein from the extracellular enveloped virus (EEV) form of vaccinia virus. BALB/c mice were immunized with Escherichia coli-produced A27L, D8L, and B5R proteins in an adjuvant consisting of monophosphoryl lipid A and trehalose dicorynomycolate or in TiterMax Gold adjuvant. Following immunization, mice were either sacrificed for analysis of immune responses or lethally challenged by intranasal inoculation with vaccinia virus strain Western Reserve. We observed that three immunizations either with A27L, D8L, and B5R or with the A27L and B5R proteins alon...Continue Reading

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Citations

Mar 31, 2010·The Journal of Infectious Diseases·Michael S SeamanRaphael Dolin
Mar 31, 2010·The Journal of Infectious Diseases·Marissa B WilckRaphael Dolin
Sep 16, 2009·Antimicrobial Agents and Chemotherapy·Aklile BerhanuDouglas W Grosenbach
Jul 28, 2010·PloS One·José M Aguilar-YáñezMario M Alvarez
Apr 6, 2011·Research in Veterinary Science·Hartwig P HuemerNorbert Nowotny
Dec 7, 2010·Immunobiology·Hartwig P HuemerMarion Pavlic
Jun 16, 2009·Current Opinion in Immunology·Richard B KennedyGregory A Poland
Aug 19, 2008·Vaccine·Carla PortocarreroMaxim Golovkin
Jan 5, 2011·Immunological Reviews·Bernard Moss
Jun 12, 2013·Biotechnology Progress·Pamela B Sánchez-ArreolaMario M Alvarez
Apr 7, 2016·Expert Review of Vaccines·Emily A VoigtGregory A Poland
Jun 25, 2016·Viruses·Olga V ChervyakovaRosemarie W Hammond
May 21, 2016·Proteomics. Clinical Applications·Philipp KuhnMichael Hust
Aug 3, 2011·Expert Review of Vaccines·Joseph W Golden, Jay W Hooper
Jul 27, 2021·Frontiers in Cellular and Infection Microbiology·Kristian Daniel Ralph RothMichael Hust

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