Vaccination with a live attenuated Acinetobacter baumannii deficient in thioredoxin provides protection against systemic Acinetobacter infection

Vaccine
Sarah AinsworthBernard P Arulanandam

Abstract

Multi-drug resistant Acinetobacter baumannii (MDR-Ab), an opportunistic pathogen associated with nosocomial and combat related infections, has a high mortality due to its virulence and limited treatment options. Deletion of the thioredoxin gene (TrxA) from a clinical isolate of MDR-Ab resulted in a 100-fold increase in 50% lethal dose (LD50) in a systemic challenge murine model. Thus, we investigated the potential use of this attenuated strain as a live vaccine against MDR-Ab. Mice were vaccinated by subcutaneous (s.c.) injection of 2×105 CFU of the ΔtrxA mutant, boosted 14days later with an equivalent inoculum, and then challenged 30days post-vaccination by i.p. injection with 10 LD50 of the wild type (WT) Ci79 strain. Efficacy of vaccination was evaluated by monitoring MDR-Ab specific antibody titers and cytokine production, observing pathology and organ burdens after WT challenge, and measuring levels of serum pentraxin-3, a molecular correlate of A. baumannii infection severity, before and after challenge. Mice vaccinated with ΔtrxA were fully protected against the lethal challenge of WT. However, minimal immunoglobulin class switching was observed with IgM predominating. Spleens harvested from vaccinated mice exhibited neg...Continue Reading

References

Dec 5, 1968·European Journal of Biochemistry·A Holmgren
Jan 23, 1999·Journal of Immunological Methods·A FreyD Zurakowski
Mar 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jaya K KumarCharles C Richardson
Jul 21, 2004·Pathologie-biologie·P Nordmann
Aug 19, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Robert GaynesUNKNOWN National Nosocomial Infections Surveillance System
Feb 1, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Pierre Edouard Fournier, Hervé Richet
Sep 20, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Naomi E AronsonKimberly A Moran
Mar 29, 2007·The Journal of Experimental Medicine·Sébastien JaillonAlberto Mantovani
Apr 26, 2007·Annals of Surgery·Kyle PetersenJames R Dunne
Mar 4, 2008·Expert Opinion on Pharmacotherapy·Jordi Vila, Jerónimo Pachón
Jul 16, 2008·Clinical Microbiology Reviews·Anton Y PelegDavid L Paterson
Mar 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Leah E ColeStefanie N Vogel
Feb 26, 2010·Journal of Clinical Microbiology·Maria Soledad RamirezMarcelo E Tolmasky
May 28, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Joel Fishbain, Anton Y Peleg
Oct 12, 2010·Journal of Global Infectious Diseases·Vikas ManchandaNp Singh
Jun 18, 2011·Vaccine·Michael J McConnellJerónimo Pachón
Jul 25, 2012·Infection and Immunity·Leticia V BentancorTomás Maira-Litrán
Nov 6, 2012·The Journal of Surgical Research·Jurandir J Dalle LuccaGeorge C Tsokos
Jan 1, 2014·Current Pharmaceutical Biotechnology·Meritxell Garcia-QuintanillaMichael J McConnell
Jul 9, 2014·Infection and Immunity·Patrick M KetterBernard P Arulanandam
Oct 30, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Rachel LatibeaudiereL Silvia Munoz-Price
Jul 2, 2015·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Tugba Arslan GulenMehmet Tasyaran
May 1, 2016·Applied and Environmental Microbiology·Fenella D HalsteadBeryl A Oppenheim

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Citations

Sep 13, 2019·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Zahra AghajaniSeyed Latif Mousavi Gargari
May 15, 2020·Clinical Microbiology Reviews·David M P De OliveiraMark J Walker
Jul 13, 2018·Therapeutic Advances in Vaccines and Immunotherapy·E David G McIntosh
Aug 6, 2019·Frontiers in Microbiology·Faye C MorrisAnton Y Peleg
Jan 11, 2020·Frontiers in Microbiology·Holly C MayBernard P Arulanandam
Dec 9, 2020·FEMS Microbiology Reviews·Mireia López-SilesMichael J McConnell
Feb 9, 2021·Expert Review of Vaccines·Crystal Ma, Wangxue Chen
Nov 18, 2020·International Journal of Molecular Sciences·Corneliu Ovidiu VrancianuMariana Carmen Chifiriuc
Mar 8, 2020·Microbial Pathogenesis·Sepideh FereshtehFarzad Badmasti
May 7, 2021·FEMS Microbiology Reviews·Mireia López-SilesMichael J McConnell
Jul 28, 2021·Microbial Pathogenesis·Stella Buchhorn de FreitasDaiane Drawanz Hartwig

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