Vaccination with Leishmania soluble antigen and immunostimulatory oligodeoxynucleotides induces specific immunity and protection against Leishmania donovani infection

FEMS Immunology and Medical Microbiology
Poonam TewaryR Madhubala

Abstract

In this report, we investigated the effect of ODN containing immunostimulatory CG motifs as adjuvant with soluble antigen (SA) from Leishmania donovani. BALB/c mice were vaccinated with the soluble antigen with or without CpG-ODN as adjuvant and then challenged with L. donovani metacyclic promastigotes. CpG-ODN alone resulted in partial protection against challenge with L. donovani. Immunization of mice with SA and CpG-ODN showed enhanced reduction in parasite load ( approximately 60%) when compared to SA ( approximately 40%) immunized mice. Immunization with SA by itself resulted in a mixed Th1/Th2 response whereas co-administration of SA with CpG-ODN resulted in a strong Th1 promoting isotype as they together promoted production of immunoglobulin G2a. Leishmania-specific Th1 cytokine response was induced by co-administering CpG-ODN and SA as they together promoted production of IFN-gamma and IL-12. In the present study, we demonstrate that immunostimulatory phosphorothioate-modified ODN are promising immune enhancers for vaccination against visceral leishmaniaisis.

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Citations

Sep 14, 2011·Journal of Tropical Medicine·Krystal J Evans, Lukasz Kedzierski
Oct 13, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Swati BhowmickNahid Ali
Apr 24, 2012·Journal of Parasitology Research·Marilia S FariaAna Paula C A Lima
Oct 23, 2019·Vaccines·Shubhranshu ZutshiBhaskar Saha

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