Vaccination with mannan protects mice against systemic aspergillosis

Medical Mycology
Min LiuDavid A Stevens

Abstract

Invasive aspergillosis is a major cause of mortality in immunocompromised patients and therapeutic options are often limited, thus a vaccine would be desirable. We presently studied acid-stable cell-wall mannan (α-1, 6-linked backbone highly branched with α-1, 2; α-1, 3; and β-1, 2-linked manno-oligomers) derived from C. albicans, with or without conjugation to bovine serum albumin (BSA), as a vaccine against systemic aspergillosis. Mice were vaccinated subcutaneously with mannan or mannan-BSA conjugate weekly 3 times, ending 2 weeks prior to infection with A. fumigatus conidia. Results showed that the protection induced by mannan is dose-dependent; 12 mg unconjugated mannan alone or > 0.3 mg mannan-BSA consistently enhanced survival (P < 0.05). Fungal burdens in brains and kidneys were reduced after > 0.3 mg of mannan-BSA (all P < 0.05). Mannan-induced protection was improved about 40-fold by conjugation of BSA to mannan. Mannan-BSA (500 kDa) was more protective than 40 kDa mannan-BSA. Mannan is a candidate for a cross-protective conjugate fungal vaccine.

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Citations

Jun 8, 2014·Carbohydrate Polymers·Eva MachováSlavomír Bystrický
Aug 15, 2014·Mycopathologia·Tanya MajumderDavid A Stevens
Dec 17, 2015·Glycoconjugate Journal·Pavol FarkašSlavomír Bystrický
May 23, 2015·Drug Discoveries & Therapeutics·Pingli Li, Fengshan Wang
Aug 25, 2015·Journal of Medical Microbiology·Karl V ClemonsDavid A Stevens
Nov 5, 2014·Medical Mycology·Deivys Leandro F PortuondoIracilda Z Carlos
Jun 5, 2016·Carbohydrate Polymers·Alžbeta ČížováSlavomír Bystrický
Sep 18, 2016·Medical Mycology·Stuart M Levitz
Dec 19, 2018·Journal of Fungi·Chris D LauruschkatJuergen Loeffler
Aug 28, 2020·Medical Mycology·Aryse Martins MeloMelissa Orzechowski Xavier

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