Vaccination With Mouse Dendritic Cells Loaded With an IpaD-IpaB Fusion Provides Protection Against Shigellosis

Frontiers in Immunology
Olivia ArizmendiFrancisco J Martinez-Becerra

Abstract

Diarrheal diseases are a major cause of morbidity and mortality worldwide. They are most prevalent in settings with inadequate sanitation, poor hygiene and contaminated water. An important diarrheal pathogen in such settings is Shigella. No commercially available vaccine exists against shigellosis and immunity to the pathogen is serotype-restricted. We have previously shown that a polypeptide fusion of the Type Three Secretion Apparatus (T3SA) proteins IpaB and IpaD (named DBF) was efficacious as a vaccine against Shigella. Vaccination using different administration routes indicated that protection conferred by DBF did not fully correlate with antibodies. To define the immune responses involved in protection, we studied cellular responses to intranasal immunization with the DBF and the adjuvant dmLT. We found dendritic cell (DC) activation at the nasal associated lymphoid tissue (NALT). Activation markers CD86 and MHCII significantly increase in cells from immunized mice. Antigen exposure in vitro further confirmed the upregulation of CD80 and CD40 in primary dendritic cells. Animals immunized with antigen-primed dendritic cells were protected against Shigella infection, at levels comparable to the efficacy of immunization with...Continue Reading

References

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Dec 29, 2011·Infection and Immunity·Francisco J Martinez-BecerraWendy L Picking
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Apr 14, 2016·Infection and Immunity·Olivia ArizmendiWendy L Picking

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Methods Mentioned

BETA
flow cytometry
ELISA
lavages
flow
FCS

Software Mentioned

GraphPad Prism
Meso Scale Discovery
FlowJo
FCS Express

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