Abstract
Neutrophil inhibitory factor (NIF), a protein isolated from hookworms of the genus Ancylostoma, inhibits CD11b/18-dependent leucocyte function, binding to the I domain of CD11b. Historically, NIF was serendipitously isolated from whole worm extracts during a search for novel antihaemostatic agents, and little is known of its source or biological significance to the parasite. NIF has also been identified as a possible hookworm vaccine candidate. Ancylostoma ceylanicum recombinant NIF, expressed in its active form in Pichia pastoris, was purified and its functional activity confirmed using neutrophil adhesion assays and confirmatory immunoassay. Recombinant NIF was subsequently used in vaccination trials in the A. ceylanicum-hamster model system for human hookworm infection. Vaccinated and challenged animals were not protected in terms of worm burden or haematocrit values, despite the presence of high levels of specific antibody against NIF. However, adult worms resident in vaccinated animals showed a significant reduction in fecundity (85.8% by day 21 postinfection), indicating a degree of protection against subsequent transmission by vaccination. These data indicate that targeted vaccination with recombinant subunit material, d...Continue Reading
References
Feb 1, 1979·Experimental Parasitology·J M Behnke, H A Parish
Mar 1, 1991·Transactions of the Royal Society of Tropical Medicine and Hygiene·D I PritchardA E Keymer
Nov 1, 1974·The American Journal of Tropical Medicine and Hygiene·U P Kalkofen
Jan 1, 1970·Zeitschrift Für Parasitenkunde·U P Kalkofen
Jan 1, 1971·Advances in Parasitology·T A Miller
Jun 1, 1995·Parasite Immunology·R J QuinnellD I Pritchard
Dec 1, 1994·The Journal of Cell Biology·P RieuM A Arnaout
Jul 1, 1995·International Journal for Parasitology·P M BrophyD L Pritchard
Jun 1, 1995·Parasitology·A BrownD I Pritchard
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·M CappelloP J Hotez
Feb 1, 1995·Parasite Immunology·D I PritchardE A Walsh
Jun 1, 1995·Scientific American·P J Hotez, D I Pritchard
Feb 1, 1994·International Journal for Parasitology·J M BehnkeJ Little
Mar 1, 1993·Pulmonary Pharmacology·J StolkG Steffens
May 1, 1993·Parasitology·R J QuinnellD I Pritchard
Jun 1, 1993·The Journal of Infectious Diseases·M CappelloP J Hotez
Apr 1, 1993·Parasite Immunology·A Brown, D I Pritchard
Jul 15, 1993·The New England Journal of Medicine·F A Oski
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·P StassensG P Vlasuk
Oct 9, 1999·The Journal of Infectious Diseases·K Ghosh, P J Hotez
Dec 3, 1999·Immunological Reviews·P J HotezR A Koski
Nov 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·F J CulleyT J Williams
Apr 3, 2001·Trends in Parasitology·D I Pritchard, A Brown
Citations
Jun 5, 2003·International Journal for Parasitology·John P DaltonAlex Loukas
Nov 22, 2002·Research in Veterinary Science·A P YatsudaE De Vries
Nov 29, 2007·Journal of Helminthology·L M M AlkazmiJ M Behnke
Mar 20, 2009·Journal of Helminthology·J A AldhounK Skírnisson
Jul 5, 2005·Foodborne Pathogens and Disease·Peter R DaviesFrank T Jones
May 24, 2005·Infection and Immunity·Susana MendezPeter J Hotez
Oct 7, 2003·Annual Review of Phytopathology·Douglas P JasmerGeert Smant
Feb 26, 2008·BMC Genomics·Iain W ChalmersKarl F Hoffmann
Nov 10, 2013·PloS One·Alexander A VeenstraTimothy S Kern
Dec 20, 2008·Molecular and Biochemical Parasitology·Xiao-Jun WuTimothy P Yoshino
Mar 18, 2005·Parasite Immunology·R J QuinnellD I Pritchard
Mar 4, 2008·Parasite Immunology·R D BungiroM Cappello
Dec 19, 2007·Parasite Immunology·K A Anbu, P Joshi
Jan 1, 2010·Parasite Immunology·E T Cadman, R A Lawrence
Jul 29, 2015·Parasite Immunology·C C ConstantinoiuJ L Scott
Aug 5, 2015·Biotechnology Advances·Namitha MohandasRobin B Gasser
Oct 31, 2009·International Journal for Parasitology·Dominic Rees-RobertsMurray E Selkirk
Aug 29, 2002·The Journal of Parasitology·Peter J HotezBernard C Zook
May 17, 2019·Journal of Helminthology·S Gomez-FuentesL Adalid-Peralta