PMID: 9529077Apr 7, 1998Paper

Vaccination with plasmid DNA encoding mycobacterial antigen 85A stimulates a CD4+ and CD8+ T-cell epitopic repertoire broader than that stimulated by Mycobacterium tuberculosis H37Rv infection

Infection and Immunity
O DenisK Huygen

Abstract

Vaccination of mice with plasmid DNA carrying the gene for the major secreted mycobacterial antigen 85A (Ag85A) from Mycobacterium tuberculosis is a powerful technique for generating robust specific Thl helper T-cell responses, CD8+-mediated cytotoxicity, and protection against M. tuberculosis challenge (K. Huygen et al., Nat. Med. 2:893-898, 1996). We have now analyzed in more detail the antigen-specific immune CD4+- and CD8+-T-cell responses induced in BALB/c mice vaccinated with Ag85A DNA and have compared these responses to those generated by intravenous infection with M. tuberculosis. T-cell-epitope mapping, as measured by interleukin-2 and gamma interferon secretion from splenic T cells restimulated in vitro with synthetic 20-mer peptides spanning the complete mature sequence of Ag85A, demonstrated that DNA vaccination stimulated a stronger and broader T-cell response than did M. tuberculosis infection. Moreover, elevated cytotoxic T lymphocyte (CTL) activity against Ag85A-transfected and peptide-pulsed P815 target cells could be generated exclusively by vaccination with plasmid DNA, not following M. tuberculosis infection. By using DNA vaccination, three Ag85A CTL epitopes with predicted major histocompatibility complex ...Continue Reading

References

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Citations

Apr 27, 2000·Current Opinion in Pulmonary Medicine·T M Doherty, P Andersen
Dec 20, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S M SmithH M Dockrell
Jun 18, 2002·Infection and Immunity·S D'SouzaK Huygen
Mar 26, 2003·Infection and Immunity·Kris Huygen
Aug 19, 2006·Clinical and Vaccine Immunology : CVI·Archna SharmaSujoy K Das Gupta
Apr 9, 2008·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Mikako HiuraYuji Hinoda
May 29, 2019·European Journal of Immunology·Shihoko Komine-AizawaMitsuo Honda
Nov 24, 2004·The Journal of Experimental Medicine·Arati B KamathSamuel M Behar
Oct 13, 2006·Current Opinion in Infectious Diseases·R F BreimanP M McInnes
Jul 31, 2007·Future Microbiology·Kris Huygen
Jan 24, 2014·Scandinavian Journal of Infectious Diseases·Bruno Rivas-Santiago, Alberto R Cervantes-Villagrana
May 9, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R N ColerM R Alderson
Aug 18, 2000·Immunology and Cell Biology·S M Smith, H M Dockrell
Jul 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nadia CaccamoAlfredo Salerno
Jun 5, 2007·Expert Review of Vaccines·Samuel M BeharYing Wu

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