Vaccination with the glycoprotein D gene of pseudorabies virus delivered by nonpathogenic Escherichia coli elicits protective immune responses

Vaccine
A L ShiauC L Wu

Abstract

Attenuated intracellular bacteria, such as Salmonella and Shigella, have been exploited to act as gene delivery vectors. In this study, we report that nonpathogenic, live Escherichia coli can be used for the delivery of DNA vaccines in vivo, leading to generation of immune responses against plasmid-encoded foreign antigens. The pseudorabies virus (PrV) DNA vaccine carrying the glycoprotein D (gD) gene delivered by E. coli was able to induce protective immune responses in mice against a lethal PrV challenge. Co-delivery of E. coli carrying plasmid DNA encoding prothymosin alpha enhanced cellular immune responses to the PrV DNA vaccine delivered by E. coli. Our results suggest that nonpathogenic E. coli may be used as a vector for DNA vaccines in veterinary uses.

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Citations

Dec 20, 2005·The Journal of Pathology·Georges VassauxNick R Lemoine
Mar 5, 2004·Expert Opinion on Biological Therapy·Holger Loessner, Siegfried Weiss
Nov 10, 2004·International Journal of Medical Microbiology : IJMM·Christoph SchoenSabine Pilgrim
Apr 14, 2006·Veterinary Research·Volker GerdtsLorne A Babiuk
Oct 12, 2019·Veterinary Research·Seyed Davoud Jazayeri, Chit Laa Poh
Oct 8, 2013·Molecular Pharmaceutics·Charles H JonesBlaine A Pfeifer

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