Vaccine efficacy of Salmonella strains expressing glycoprotein 63 with different promoters.

Infection and Immunity
S J McSorleyF Y Liew

Abstract

The development of Salmonella vaccine vectors has been hindered by both the requirement for multiple doses to induce immune responses and a lack of plasmid stability. Direct comparisons of different promoter systems with the same antigen are necessary to address these important issues. We have previously described an AroA- AroD- deletion mutant of Salmonella typhimurium (GID101) which expresses the gene encoding the Leishmania major promastigote surface glycoprotein gp63 (GID101). While this construct provided significant protection against L. major challenge to highly susceptible BALB/c mice, this required at least two oral doses. We report here the use of two different inducible promoters, the nirB and osmC promoters, to improve vaccine efficacy. These constructs (termed GID105 and GID106, respectively) expressed gp63 in vitro under inducible conditions and colonized BALB/c mice after oral administration. GID105 demonstrated greater plasmid stability in vitro and in vivo than did either GID106 or GID101, which expresses gp63 constitutively. Spleen and lymph node cells from mice immunized with a single oral dose of GID105 proliferated in vitro in response to L. major and secreted gamma interferon, whereas cells from mice given...Continue Reading

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Citations

Mar 28, 1998·The Journal of Experimental Medicine·D XuF Y Liew
Sep 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aparna SrinivasanStephen J McSorley
Oct 6, 2004·International Journal of Immunopathology and Pharmacology·P TsagozisE Dotsika
Oct 3, 2002·American Journal of Clinical Dermatology·Peter C Melby
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Dec 24, 2002·Infection and Immunity·Saeed AhmedDiane McMahon-Pratt
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S J McSorleyM K Jenkins
Jun 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·Maria ColmenaresDiane McMahon-Pratt
Feb 2, 2013·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C Teixeira, R Gomes
Sep 14, 2011·Journal of Tropical Medicine·Krystal J Evans, Lukasz Kedzierski
Jun 20, 2014·Immunological Reviews·Stephen J McSorley
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Oct 11, 2008·Expert Review of Vaccines·Pascal LaunoisMarie-Paul Kieny
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May 28, 2011·International Immunopharmacology·Rajeev Nagill, Sukhbir Kaur

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