Vaccine engineering: enhancement of immunogenicity of synthetic peptide vaccines related to hepatitis in chemically defined models consisting of T- and B-cell epitopes.

Proceedings of the National Academy of Sciences of the United States of America
J P Tam, Y A Lu

Abstract

We report the development of two models for synthetic hepatitis B vaccines. The models were based on the multiple antigen peptide (MAP) system and contained the relevant B- and T-cell epitopes without any macromolecular carrier. Two peptides, representing the a determinant of the S region (S protein) of hepatitis B surface antigen, a dominant serotype of hepatitis B virus infection found in humans, and residues 12-26 of the pre-S(2) region of the middle protein were incorporated as either monoepitope or diepitope MAP models. Immunizations of outbred rabbits with the monoepitope MAP that contains the pre-S(2) antigen resulted in high-titered antibody response to the middle protein, but the other monoepitope, containing only the a-determinant peptide antigen, resulted in poor immune responses to either the peptide antigens or to the S protein. The diepitope MAPs containing both the a and the pre-S(2) determinants produced high-titer antibodies reactive to the a-synthetic peptide and the S protein, as well as to the middle proteins. Thus, our results show that the diepitope MAP models eliminate the need for a protein carrier and that the pre-S(2) peptide determinant serves as a T-helper cell epitope that enhances the immune respon...Continue Reading

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Citations

Jun 11, 1999·Journal of Peptide Science : an Official Publication of the European Peptide Society·P Veprek, J Jezek
May 24, 2000·Journal of Peptide Science : an Official Publication of the European Peptide Society·L J CruzO Reyes
Aug 27, 1998·Electrophoresis·B T KurienR H Scofield
Feb 14, 2009·Cellular and Molecular Neurobiology·Eva KontsekovaMichal Novak
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Jun 20, 2002·Journal of Biotechnology·Kristen Sadler, James P Tam
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Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M K HartB F Haynes
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May 24, 2011·International Immunology·Esther J WitschMichael Sela
Dec 17, 2008·Protein Engineering, Design & Selection : PEDS·Michael J Sweredoski, Pierre Baldi
Aug 30, 2005·Antimicrobial Agents and Chemotherapy·Mougli SuarezNicolas Mermod

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