PMID: 8970979Dec 1, 1996Paper

Vaccinia virions lacking the RNA helicase nucleoside triphosphate phosphohydrolase II are defective in early transcription

Journal of Virology
C H Gross, S Shuman

Abstract

Temperature-sensitive mutations (ts10, ts18, and ts39) of the vaccinia virus RNA helicase nucleoside triphosphate phosphohydrolase II (NPH-II) result in the production of noninfectious progeny virions at the restrictive temperature. The noninfectious mutant particles contain the wild-type complement of virion core and envelope polypeptides, as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The results of Western blot (immunoblot) analysis indicate that these particles lack NPH-II, whereas other enzymatic components of the virus core are present. These components include the following: DNA-dependent RNA polymerase subunits rpo147, rpo132, rpo94, rpo35, rpo30, rpo22, and rpo18; early transcription initiation factor subunits A8 and D6; mRNA capping enzyme subunits D1 and D12; RNA cap 2'-O-methyltransferase; A18 DNA helicase; DNA-dependent ATPase NPH-I; and DNA topoisomerase. Although RNA polymerase is encapsidated by the mutant viruses, mRNA synthesis in vitro by permeabilized mutant virions is only 5 to 20% that of the wild-type virus, as judged by nucleoside monophosphate incorporation into acid-insoluble material. Moreover, the transcripts synthesized by the mutant particles are longer than normal and rema...Continue Reading

References

Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B S SchnierleB Moss
Apr 1, 1992·The Journal of General Virology·E V Koonin, T G Senkevich
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S Shuman
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Apr 1, 1996·Journal of Virology·C H Gross, S Shuman
Sep 1, 1962·Virology·W K JOKLIK

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Citations

Jul 1, 1999·Protein Science : a Publication of the Protein Society·A Y HoweJ Y Lau
Nov 25, 2005·Journal of Virology·Dawn EckertMichael Merchlinsky
Aug 29, 2006·Nucleic Acids Research·Kuan-Teh Jeang, Venkat Yedavalli
Dec 22, 2010·RNA Biology·Arnaz Ranji, Kathleen Boris-Lawrie
Jan 30, 2010·The Journal of Biological Chemistry·Sean David TaylorAnna Marie Pyle
Jul 26, 2012·The Journal of Biological Chemistry·Zhilong YangBernard Moss
Jan 29, 2013·RNA Biology·Sebastian L B KönigDavid Rueda
Jun 16, 2012·RNA Biology·Lenz Steimer, Dagmar Klostermeier
Sep 15, 2006·Journal of Virology·Patricia RenestoJean-Michel Claverie
May 11, 1999·Journal of Virology·O WilliamsM Merchlinsky

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