PMID: 9445029Jan 28, 1998Paper

Vaccinia virus 15-kilodalton (A14L) protein is essential for assembly and attachment of viral crescents to virosomes

Journal of Virology
J F RodriguezD Rodriguez

Abstract

Early stages in vaccinia virus (VV) assembly involve the recruitment of cellular membranes from the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) to virus factories (or virosomes). The key viral factors involved in this process are not yet known. We have previously identified and characterized two viral proteins, of 21 kDa (A17L gene) and 15 kDa (A14L gene), that associate with tubulovesicular elements related to the ERGIC and are localized in viral membranes at all stages of virion assembly. We showed that the 21-kDa protein is not responsible for the recruitment of membranes from the ERGIC to viral factories. However, it appears to be essential for the organization of viral membranes. In this investigation we have generated a VV recombinant, VVindA14L, in which the expression of the A14L gene is inducibly regulated by the Escherichia coli lacI operator-repressor system. Repression of 15-kDa protein synthesis has a dramatic effect on virus yields and severely impairs plaque formation. Compared to wild-type VV, reduced amounts of 15-kDa protein are produced in VVindA14L-infected cells in the presence of IPTG (isopropyl-beta-D-thiogalactoside), and this correlates with a small-plaque phenotype and reduced VVindA14...Continue Reading

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Citations

Jul 15, 2005·Journal of Virology·Alan C TownsleyBernard Moss
May 14, 2005·Journal of Virology·Jason Mercer, Paula Traktman
May 19, 2011·Archives of Virology·Petr ChlandaJacomine Krijnse-Locker
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·S McCraithS Fields
Jul 29, 2004·Journal of Virology·Bethany Unger, Paula Traktman
Feb 14, 2004·Journal of Virology·Tatiana G SenkevichBernard Moss
Dec 17, 2008·Biology of the Cell·Francisco Javier ChichónJosé L Carrascosa
Dec 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Matloob HusainBernard Moss
Apr 1, 2010·Viruses·Jason P Laliberte, Bernard Moss
Aug 22, 2007·Cellular Microbiology·Grazyna KochanMariano Esteban
Nov 3, 2014·Annual Review of Virology·Cristina RiscoSriram Subramaniam
Jan 14, 2011·Journal of Virology·Liliana Maruri-AvidalBernard Moss

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