Vaccinia virus DNA ligase recruits cellular topoisomerase II to sites of viral replication and assembly.

Journal of Virology
Y-C James LinD H Evans

Abstract

Vaccinia virus replication is inhibited by etoposide and mitoxantrone even though poxviruses do not encode the type II topoisomerases that are the specific targets of these drugs. Furthermore, one can isolate drug-resistant virus carrying mutations in the viral DNA ligase and yet the ligase is not known to exhibit sensitivity to these drugs. A yeast two-hybrid screen was used to search for proteins binding to vaccinia ligase, and one of the nine proteins identified comprised a portion (residue 901 to end) of human topoisomerase IIbeta. One can prevent the interaction by introducing a C(11)-to-Y substitution mutation into the N terminus of the ligase bait protein, which is one of the mutations conferring etoposide and mitoxantrone resistance. Coimmunoprecipitation methods showed that the native ligase and a Flag-tagged recombinant protein form complexes with human topoisomerase IIalpha/beta in infected cells and that this interaction can also be disrupted by mutations in the A50R (ligase) gene. Immunofluorescence microscopy showed that both topoisomerase IIalpha and IIbeta antigens are recruited to cytoplasmic sites of virus replication and that less topoisomerase was recruited to these sites in cells infected with mutant virus ...Continue Reading

References

Nov 25, 1989·Nucleic Acids Research·S M Kerr, G L Smith
Aug 14, 1971·Journal of Molecular Biology·D M PrescottJ B Kirkpatrick
Jan 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·J SekiguchiS Shuman
Feb 15, 1997·Nucleic Acids Research·J Sekiguchi, S Shuman
Sep 28, 1998·Biochimica Et Biophysica Acta·S Shuman
Jun 8, 2001·Annual Review of Biochemistry·J J Champoux
Apr 1, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sunil J AdvaniBernard Roizman
May 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yi Lisa Lyu, James C Wang
Sep 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Flavio Da Fonseca, Bernard Moss
Aug 1, 1961·The Journal of Biophysical and Biochemical Cytology·S DALES, L SIMINOVITCH
May 14, 2004·Annual Review of Biophysics and Biomolecular Structure·Kevin D Corbett, James M Berger
Sep 24, 2004·Current Medicinal Chemistry·Philippe MeresseEmmanuel Bertounesque
Sep 29, 2005·Journal of Virology·Jaewook Oh, Steven S Broyles
Mar 21, 2006·The Journal of Biological Chemistry·Hung-Ji TsaiShu-Chun Teng
Aug 4, 2006·Molecular Cell·Kay PerryGregory D Van Duyne
Sep 25, 2007·The Journal of Biological Chemistry·Matthew J CulybaFrederic D Bushman

❮ Previous
Next ❯

Citations

Feb 15, 2013·Journal of Virology·Wondimagegnehu M TeferiDavid H Evans
Apr 20, 2012·Journal of Virology·Chad R Irwin, David H Evans
Dec 1, 2009·Microbiology and Molecular Biology Reviews : MMBR·Kim Van VlietGrant McFadden
Feb 16, 2016·Archives of Biochemistry and Biophysics·Anil ChekuriAnand K Kondapi
Dec 21, 2011·Antiviral Research·Sharon E AltmannJoseph E Blaney
May 30, 2009·Journal of Virological Methods·Wendy C MageeDavid H Evans
Jul 8, 2014·Virology·Venkata ChelikaniKiran Kondabagil
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Gilad SivanBernard Moss
Apr 27, 2016·Nucleic Acids Research·Darius KazlauskasČeslovas Venclovas
Aug 16, 2016·PLoS Pathogens·Patrick PaszkowskiDavid H Evans
Oct 4, 2017·Molecular & Cellular Proteomics : MCP·Emigdio D ReyesMatthew D Weitzman
Apr 21, 2018·The Journal of General Virology·Rutger David LuteijnEmmanuel J H J Wiertz
Jan 15, 2014·Cell Death and Differentiation·S A H de Poot, N Bovenschen
Mar 23, 2017·Proceedings of the National Academy of Sciences of the United States of America·Debasis PandaBernard Moss
Feb 6, 2020·Proceedings of the National Academy of Sciences of the United States of America·Chen Peng, Bernard Moss
Jul 30, 2009·Journal of Proteome Research·Leiliang ZhangGrant McFadden

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.