PMID: 7545242Oct 1, 1995Paper

Vaccinia virus gene A18R encodes an essential DNA helicase

Journal of Virology
D A Simpson, R C Condit

Abstract

The vaccinia virus A18R protein is a DNA-dependent ATPase that contains the canonical sequence motifs associated with the DEXH group of DNA and RNA helicases. Investigation of A18R protein function during infection indicated it functions in the early and late phases of vaccinia virus transcription. The A18R protein shares sequence similarity with the mammalian DNA helicase ERCC3. The ERCC3 protein has a dual function: it is a component of the transcription factor TFIIH and is an essential participant in the cellular nucleotide excision repair pathway. Here we present evidence that the A18R protein is a DNA helicase that unwinds duplex DNA in a 3'-to-5' direction. The A18R helicase was inactive on RNA-DNA and RNA-RNA hybrids. The A18R unwinding activity was most efficient on DNA substrates with lengths of 20 nucleotides or less, and its unwinding activity was not stimulated by the addition of Escherichia coli single-strand-binding protein (SSB), the bacteriophage T4 gene 32 SSB, or the vaccinia virus I3L protein, a putative SSB. We have used an electrophoretic gel mobility shift assay to show that the A18R protein forms a stable complex with single-stranded DNA, and to a lesser extent RNA, in a reaction that does not require ATP.

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Citations

Jan 11, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Fumitaka MizukiTokio Tani
Jul 24, 2010·Archives of Virology·Fong-Yuan LinMin-Liang Wong
Aug 15, 2003·The Journal of General Virology·Steven S Broyles
May 7, 2004·European Journal of Biochemistry·Narendra Tuteja, Renu Tuteja
Dec 16, 1998·Journal of Virology·C L AfonsoD L Rock
Jan 8, 2000·The Journal of Biological Chemistry·C A Lackner, R C Condit
Jan 20, 2000·Virology·S N ShchelkunovJ J Esposito
Jun 27, 2002·Virology·S N ShchelkunovB Moss
Sep 6, 2002·Biochimica Et Biophysica Acta·Richard C Condit, Edward G Niles
Apr 1, 1996·Journal of Virology·C H Gross, S Shuman
Apr 15, 2005·Journal of Medicinal Chemistry·Michael C PirrungEarl R Kern

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