Vaccinia virus mutants with alanine substitutions in the conserved G5R gene fail to initiate morphogenesis at the nonpermissive temperature

Journal of Virology
F G da FonsecaBernard Moss

Abstract

The initial characterization of the product of the vaccinia virus G5R gene, which is conserved in all poxviruses sequenced to date, is described. The G5 protein was detected in the core fraction of purified virions, and transcription and translation of the G5R open reading frame occurred early in infection, independently of DNA replication. Attempts to delete the G5R gene and isolate a replication-competent virus were unsuccessful, suggesting that G5R encodes an essential function. We engineered vaccinia virus mutants with clusters of charged amino acids changed to alanines and determined that several were unable to replicate at 40 degrees C but grew well at 37 degrees C. At the nonpermissive temperature, viral gene expression and DNA replication and processing were unperturbed. However, tyrosine phosphorylation and proteolytic cleavage of the A17 membrane protein and proteolytic cleavage of core proteins were inhibited at 40 degrees C, suggesting an assembly defect. The cytoplasm of cells that had been infected at the nonpermissive temperature contained large granular areas devoid of cellular organelles or virus structures except for occasional short crescent-shaped membranes and electron-dense lacy structures. The temperature...Continue Reading

References

Oct 1, 1992·Genetics·K F WertmanD Botstein
May 1, 1992·Journal of Virology·S LinS S Broyles
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·Y F Zhang, B Moss
Jun 1, 1990·Journal of Virology·F G Falkner, B Moss
Dec 20, 1989·Journal of Molecular Biology·A J Davison, B Moss
Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·E Katz, B Moss
Dec 1, 1981·Cell·R W Moyer, R L Graves
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·B M BaroudyB Moss
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·S Lin, S S Broyles
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·D E Hassett, R C Condit
Jan 9, 1999·Journal of Virology·M HollinsheadD J Vaux
Oct 18, 2000·Proceedings of the National Academy of Sciences of the United States of America·T G SenkevichB Moss
Jul 14, 2001·Molecular Biology of the Cell·N TolonenJ Krijnse Locker
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tatiana G SenkevichBernard Moss
Aug 7, 2002·Journal of Virology·Chelsea M ByrdDennis E Hruby
Jun 14, 2003·Journal of Virology·Chris UptonRachel L Roper

❮ Previous
Next ❯

Citations

Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Tatiana G SenkevichBernard Moss
Oct 6, 2006·Bioinformatics·Melissa Da SilvaChris Upton
Jan 14, 2011·Journal of Virology·Liliana Maruri-AvidalBernard Moss
Dec 1, 2009·Microbiology and Molecular Biology Reviews : MMBR·Kim Van VlietGrant McFadden
Nov 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Frank S De SilvaBernard Moss
Dec 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Matloob HusainBernard Moss
Feb 24, 2006·Virus Research·Lakshminarayan M IyerL Aravind
Sep 29, 2006·Virology·Wolfgang ReschBernard Moss
Apr 1, 2010·Viruses·Jason P Laliberte, Bernard Moss
Aug 26, 2016·Journal of Virology·Tuan NgoPaul David Gershon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.