Vaccinia virus protein complex F12/E2 interacts with kinesin light chain isoform 2 to engage the kinesin-1 motor complex

PLoS Pathogens
David C J CarpentierGeoffrey L Smith

Abstract

During vaccinia virus morphogenesis, intracellular mature virus (IMV) particles are wrapped by a double lipid bilayer to form triple enveloped virions called intracellular enveloped virus (IEV). IEV are then transported to the cell surface where the outer IEV membrane fuses with the cell membrane to expose a double enveloped virion outside the cell. The F12, E2 and A36 proteins are involved in transport of IEVs to the cell surface. Deletion of the F12L or E2L genes causes a severe inhibition of IEV transport and a tiny plaque size. Deletion of the A36R gene leads to a smaller reduction in plaque size and less severe inhibition of IEV egress. The A36 protein is present in the outer membrane of IEVs, and over-expressed fragments of this protein interact with kinesin light chain (KLC). However, no interaction of F12 or E2 with the kinesin complex has been reported hitherto. Here the F12/E2 complex is shown to associate with kinesin-1 through an interaction of E2 with the C-terminal tail of KLC isoform 2, which varies considerably between different KLC isoforms. siRNA-mediated knockdown of KLC isoform 1 increased IEV transport to the cell surface and virus plaque size, suggesting interaction with KLC isoform 1 is somehow inhibitory...Continue Reading

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Citations

Oct 16, 2015·Seminars in Cell & Developmental Biology·Timothy P Newsome, N Bishara Marzook
Sep 22, 2017·The Journal of General Virology·David C J CarpentierGeoffrey L Smith
Dec 8, 2020·The Journal of Cell Biology·Yusuke FukudaRosalind A Segal

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Datasets Mentioned

BETA
GTX114510

Methods Mentioned

BETA
immunoprecipitations
immunoprecipitation
electron microscopy
confocal microscopy
co-immunoprecipitations
transfection
flow cytometry
Co-immunoprecipitation
pull-down
PCR

Software Mentioned

ClustalW2
Zen
ImageJ
Axiovision
GraphPad Prism
Zeiss AxioVision
Photoshop
Summit

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