Abstract
Vacquinol‑1 (Vacq), a quinolone derivative, has recently been reported to display potent antitumor effects in glioblastomas by inducing cellular massive vacuolization and cell death. However, whether Vacq induces cytotoxicities in other types of cancers, and the potential underlying mechanism, remain to be investigated. In the present study, it was revealed that Vacq suppressed cell growth and colony formation in the hepatocellular carcinoma (HCC) cell lines BEL7402 and Huh7. In addition, treatment with Vacq increased the number of early and late apoptotic cells as assessed by flow cytometry with fluorescein isothiocyanate‑conjugated Annexin V and propidium iodide double staining. Notably, the effect by Vacq in the tested cells could be inhibited by pretreatment with a broad specificity caspase inhibitor Z‑VAD‑FMK, suggesting that Vacq may induce apoptosis in HCC cells. Morphologically, exposure to Vacq resulted in nuclear fragmentation and the apoptotic body formation in HCC cells. Furthermore, Vacq treatment increased the cleavage of caspase‑3, caspase‑9 and poly(adenosine diphosphate‑ribose) polymerase‑1. Mechanistic analysis revealed that Vacq upregulated the expressions of pro‑apoptotic proteins [B‑cell lymphoma 2 (bcl‑2)‑a...Continue Reading
References
Mar 22, 2002·Molecular Biology of the Cell·Marek LosKlaus Schulze-Osthoff
Mar 23, 2011·Immunology and Cell Biology·Jet Phey Lim, Paul A Gleeson
Jun 7, 2011·Molecular Cancer·Jean H OvermeyerWilliam A Maltese
Nov 14, 2012·Nature Reviews. Gastroenterology & Hepatology·Augusto VillanuevaJosep M Llovet
Dec 10, 2013·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Hiroyuki NaitohTohru Tani
Dec 21, 2013·Nature Reviews. Molecular Cell Biology·Peter E CzabotarJerry M Adams
Jan 24, 2014·Journal of Cellular Biochemistry·Laura A Gillies, Tomomi Kuwana
May 31, 2014·Cell Research·Yufeng ShiLuis F Parada
Jul 1, 2015·Oncotarget·Jiayi WangFenyong Sun
Jan 21, 2016·Cancer Science·Zeynep Firtina KaragonlarNeşe Atabey
Sep 9, 2016·Journal of Medicinal Chemistry·Lars G J HammarströmPatrik Ernfors
Nov 9, 2016·PloS One·Ryohei MiyazakiKazumasa Orihashi
Apr 15, 2017·Oncotarget·Philip SanderMarion Schneider
Jul 22, 2017·Molecular Medicine Reports·Xu WangLei Wang