Vacuolar processing enzyme plays an essential role in the crystalline structure of glutelin in rice seed

Plant & Cell Physiology
Toshihiro KumamaruHikaru Satoh

Abstract

To identify the function of genes that regulate the processing of proglutelin, we performed an analysis of glup3 mutants, which accumulates excess amounts of proglutelin and lack the vacuolar processing enzyme (VPE). VPE activity in developing seeds from glup3 lines was reduced remarkably compared with the wild type. DNA sequencing of the VPE gene in glup3 mutants revealed either amino acid substitutions or the appearance of a stop codon within the coding region. Microscopic observations showed that alpha-globulin and proglutelin were distributed homogeneously within glup3 protein storage vacuoles (PSVs), and that glup3 PSVs lacked the crystalline lattice structure typical of wild-type PSVs. This suggests that the processing of proglutelin by VPE in rice is essential for proper PSV structure and compartmentalization of storage proteins. Growth retardation in glup3 seedlings was also observed, indicating that the processing of proglutelin influences early seedling development. These findings indicate that storage of glutelin in its mature form as a crystalline structure in PSVs is required for the rapid use of glutelin as a source of amino acids during early seedling development. In conclusion, VPE plays an important role in the...Continue Reading

References

Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M P ScottN C Nielsen
Apr 1, 1989·The Plant Cell·C D DickinsonN C Nielsen
Nov 1, 1982·Archives of Biochemistry and Biophysics·A C Brinegar, D M Peterson
Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Jul 15, 1996·European Journal of Biochemistry·H Kato, T Minamikawa
May 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Motoyasu AdachiShigeru Utsumi
Jun 12, 2003·The Journal of Biological Chemistry·Tomoo ShimadaIkuko Hara-Nishimura
Oct 1, 1982·Plant Physiology·H YamagataZ Kasai
Oct 1, 1987·Plant Physiology·I Hara-Nishimura, M Nishimura
Jan 22, 2009·The Plant Journal : for Cell and Molecular Biology·Yihua WangJianmin Wan
Jul 1, 1988·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·T KumamaruK Tanaka
Mar 1, 1981·Plant Molecular Biology·C SenguptaD P Verma

❮ Previous
Next ❯

Citations

May 15, 2013·Journal of Experimental Botany·Israel JuliánManuel Martinez
Apr 28, 2011·Plant & Cell Physiology·Ai NagamineToshihiro Kumamaru
Nov 22, 2011·Plant & Cell Physiology·Kenji YamadaMikio Nishimura
Apr 24, 2014·BMC Plant Biology·Mireia BundóMaría Coca
Nov 5, 2013·International Journal of Cell Biology·Yayoi Onda
Jun 27, 2012·Functional & Integrative Genomics·C OsorioS Rustgi
Jun 21, 2011·Plant Science : an International Journal of Experimental Plant Biology·Tomokazu UshijimaToshihiro Kumamaru
Dec 22, 2010·Journal of Proteomics·Ján A Miernyk, Martin Hajduch
Nov 9, 2012·The Plant Journal : for Cell and Molecular Biology·Johana C Misas-VillamilRenier A L van der Hoorn
Nov 25, 2011·Plant Signaling & Behavior·Yayoi Onda, Yasushi Kawagoe
Nov 5, 2019·The New Phytologist·Kenji YamadaIkuko Hara-Nishimura
Feb 24, 2011·The Plant Cell·Francisca C ReyesMarisa S Otegui
May 16, 2019·Plant Science : an International Journal of Experimental Plant Biology·Hong-Li ChouThomas W Okita
Sep 29, 2021·Journal of Integrative Plant Biology·Wei HeFeng Yu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.