Vacuolar protein sorting receptor in Schizosaccharomyces pombe

Microbiology
Tomoko IwakiKaoru Takegawa

Abstract

The mechanism by which soluble proteins, such as carboxypeptidase Y, reach the vacuole in Saccharomyces cerevisiae is very similar to the mechanism of lysosomal protein sorting in mammalian cells. Vps10p is a receptor for transport of soluble vacuolar proteins in S. cerevisiae. vps10(+), a gene encoding a homologue of S. cerevisiae PEP1/VPS10, has been identified and deleted from the fission yeast Schizosaccharomyces pombe. Deletion of the vps10(+) gene resulted in missorting and secretion of Sch. pombe vacuolar carboxypeptidase Cpy1p, indicating that it is required for targeting Cpy1p to the vacuole. Sch. pombe Vps10p (SpVps10p) is a type I transmembrane protein and its C-terminal cytoplasmic tail domain is essential for Cpy1p transport to the vacuole. Cells expressing green fluorescent protein-tagged SpVps10p produced a punctate pattern of fluorescence, indicating that SpVps10p was largely localized in the Golgi compartment. In addition, Sch. pombe vps26(+), vps29(+) and vps35(+), encoding homologues of the S. cerevisiae retromer components VPS26, VPS29 and VPS35, were identified and deleted. Fluorescence microscopy demonstrated that SpVps10p mislocalized to the vacuolar membrane in these mutants. These results indicate that ...Continue Reading

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Citations

Feb 9, 2010·Applied Microbiology and Biotechnology·Alimjan IdirisKaoru Takegawa
Dec 15, 2010·Applied Microbiology and Biotechnology·Hiroyuki MukaiyamaKaoru Takegawa
Nov 23, 2011·The Journal of Cell Biology·Lei WenWen-Cheng Xiong
Feb 20, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Thi Thuy Trang NguyenEe Sin Chen
Dec 29, 2009·FEBS Letters·Hiroyuki MukaiyamaKaoru Takegawa
Jan 23, 2016·Scientific Reports·Thi Thuy Trang NguyenEe Sin Chen
Apr 17, 2012·Trends in Plant Science·David G RobinsonCorrado Viotti
Mar 25, 2014·Natural Products and Bioprospecting·Joel HeislerRadha Pyati
Jul 16, 2011·Journal of Bioscience and Bioengineering·Akira HosomiKaoru Takegawa
Jun 18, 2009·Biotechnology and Applied Biochemistry·Kaoru TakegawaHiromichi Kumagai
Sep 9, 2011·Traffic·Miguel EstravísPilar Pérez
Dec 18, 2012·The FEBS Journal·Li XiangWim Van den Ende
Jan 15, 2010·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Takumi MikawaFuyuki Ishikawa
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Feb 8, 2008·Bioscience, Biotechnology, and Biochemistry·Akira HosomiKaoru Takegawa
Sep 26, 2017·DNA and Cell Biology·Anbo GaoYuncheng Lv
Nov 20, 2013·Molecular and Cellular Biology·Evelin YoungEric C Chang
Feb 8, 2007·Bioscience, Biotechnology, and Biochemistry·Tomoko IwakiKaoru Takegawa
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Sep 13, 2020·Applied Microbiology and Biotechnology·Takao OhashiKaoru Takegawa
Dec 7, 2006·Journal of Cell Science·Thomas WassmerPeter J Cullen

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