Vacuolar sulfate transporters are essential determinants controlling internal distribution of sulfate in Arabidopsis

The Plant Cell
Tatsuhiko KataokaHideki Takahashi

Abstract

Uptake of external sulfate from the environment and use of internal vacuolar sulfate pools are two important aspects of the acquisition of sulfur for metabolism. In this study, we demonstrated that the vacuolar SULTR4-type sulfate transporter facilitates the efflux of sulfate from the vacuoles and plays critical roles in optimizing the internal distribution of sulfate in Arabidopsis thaliana. SULTR4;1-green fluorescent protein (GFP) and SULTR4;2-GFP fusion proteins were expressed under the control of their own promoters in transgenic Arabidopsis. The fusion proteins were accumulated specifically in the tonoplast membranes and were localized predominantly in the pericycle and xylem parenchyma cells of roots and hypocotyls. In roots, SULTR4;1 was constantly accumulated regardless of the changes of sulfur conditions, whereas SULTR4;2 became abundant by sulfur limitation. In shoots, both transporters were accumulated by sulfur limitation. Vacuoles isolated from callus of the sultr4;1 sultr4;2 double knockout showed excess accumulation of sulfate, which was substantially decreased by overexpression of SULTR4;1-GFP. In seedlings, the supplied [(35)S]sulfate was retained in the root tissue of the sultr4;1 sultr4;2 double knockout muta...Continue Reading

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Citations

Oct 31, 2009·Plant Cell Reports·Stanislav KoprivaAnna Koprivova
Apr 7, 2010·Amino Acids·Hankuil YiJoseph M Jez
Oct 26, 2006·Plant Molecular Biology·Hanbin DanZhi-Liang Zheng
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Nov 23, 2006·The Plant Cell·Akiko Maruyama-NakashitaHideki Takahashi
Jun 2, 2007·The Plant Cell·Franck ChopinFrançoise Daniel-Vedele
Mar 13, 2012·Annual Review of Plant Biology·Enrico MartinoiaRéka Nagy
Sep 24, 2014·Plant & Cell Physiology·María Del Carmen Rodríguez-HernándezMaría Del Carmen Martínez-Ballesta
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