Vacuum Ultraviolet Laser Desorption/Ionization Mass Spectrometry Imaging of Single Cells with Submicron Craters

Analytical Chemistry
Jia WangYuxiang Mo

Abstract

Mass spectrometry imaging (MSI) is a crucial label-free method to distinguish the localization patterns in single cells. MALDI-TOF MS and ToF-SIMS are now bearing the responsibility. However, MALDI-TOF MS is limited to micron spatial resolution and ToF-SIMS suffers from severe molecular fragmentation. Here, we proposed a new MSI methodology of vacuum ultraviolet laser desorption/ionization (VUVDI) with high spatial resolution, achieving higher ion yields and less fragmentation compared with ToF-SIMS at submicron level. The fluorescence image and mass spectrum of VUVDI were obtained simultaneously. In addition, the adjustable laser fluence acquired selective detection for different molecular and fragmental ions, thus realizing molecular identification. Furthermore, MSIs of single cells with submicron craters were presented. These results suggest VUVDI is a potential mass spectrometry method that provides a soft ionization source and submicron spatial resolution for molecular analysis in life science.

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Citations

Feb 21, 2019·Annual Review of Analytical Chemistry·Luke HanleyYeni P Yung
Nov 11, 2020·Mass Spectrometry Reviews·Vladimir A AzovAlexander A Makarov
May 25, 2021·Mass Spectrometry Reviews·Rui HuMaili Liu
Jul 11, 2021·The Review of Scientific Instruments·J Aoki, M Toyoda
Nov 23, 2019·Analytical Chemistry·Tongtong WangWei Hang
Oct 27, 2018·Analytical Chemistry·Pieter E OomenAndrew G Ewing
May 28, 2019·Analytical Chemistry·Elizabeth K NeumannJonathan V Sweedler

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