Vaginal bacterial community composition and concentrations of estradiol at the time of artificial insemination in Brangus heifers.

Journal of Animal Science
Riley D MessmanCaleb O Lemley

Abstract

The knowledge surrounding the bovine vaginal microbiota and its implications on fertility and reproductive traits remains incomplete. The objective of the current study was to characterize the bovine vaginal bacterial community and estradiol concentrations at the time of artificial insemination (AI). Brangus heifers (n = 78) underwent a 7-d Co-Synch + controlled internal drug release estrus synchronization protocol. At AI, a double-guarded uterine culture swab was used to sample the anterior vaginal tract. Immediately after swabbing the vaginal tract, blood samples were collected by coccygeal venipuncture to determine concentrations of estradiol. Heifers were retrospectively classified as pregnant (n = 29) vs. nonpregnant (n = 49) between 41 and 57 d post-AI. Additionally, heifers were classified into low (1.1 to 2.5 pg/mL; n = 21), medium (2.6 to 6.7 pg/mL; n = 30), and high (7.2 to 17.6 pg/mL; n = 27) concentration of estradiol. The vaginal bacterial community composition was determined through sequencing of the V4 region from the 16S rRNA gene using the Illumina Miseq platform. Alpha diversity was compared via ANOVA and beta diversity was compared via PERMANOVA. There were no differences in the Shannon diversity index (alpha...Continue Reading

References

Nov 1, 1978·The American Journal of Physiology·R E GarfieldE E Daniel
Jan 1, 1984·Journal of Dairy Science·G S Lewis, S K Newman
Aug 13, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Diane M Citron
Jan 26, 2008·Animal Health Research Reviews·S M DaboA W Confer
Sep 23, 2008·The Journal of Infection·Paul J Huggan, David R Murdoch
Oct 2, 2012·Microbial Pathogenesis·Katrina M HellenbrandNicole A Aulik
Jun 6, 2013·BMC Infectious Diseases·Kevin AfraDaniel Gregson
Jan 7, 2014·Seminars in Reproductive Medicine·Ido SirotaJames H Segars
Apr 15, 2014·Current Opinion in Immunology·Michael C Abt, Eric G Pamer
Jul 12, 2014·International Journal of Environmental Research and Public Health·Charlene W J AfricaMegan Stemmet
Nov 26, 2015·PloS One·Mateus Laguardia-NascimentoEdel Figueiredo Barbosa-Stancioli
Oct 21, 2016·Journal of Applied Microbiology·C Gonzalez MorenoM C Otero
Dec 23, 2016·Frontiers in Microbiology·Elizabeth A MillerElizabeth A Archie
Mar 7, 2017·Nature Reviews. Immunology·Maayan LevyEran Elinav
Jun 24, 2017·Frontiers in Microbiology·Brooke A ClemmonsPhillip R Myer
Dec 23, 2017·Geburtshilfe und Frauenheilkunde·Nina Combaz-Söhnchen, Annette Kuhn
Aug 30, 2018·Disease Models & Mechanisms·Jocelyn M WesselsCharu Kaushic
Sep 29, 2018·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Aloka B BandaraThomas J Inzana
Dec 7, 2018·Animals : an Open Access Journal From MDPI·Paula Martinez-RosAntonio Gonzalez-Bulnes
Dec 24, 2018·Research in Veterinary Science·Kiyokazu Murai, Hidetoshi Higuchi

❮ Previous
Next ❯

Citations

Mar 28, 2021·Journal of Animal Science·Riley D MessmanCaleb O Lemley
Nov 9, 2021·Frontiers in Microbiology·Lucas R KoesterStephan Schmitz-Esser

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.