Vaginal Expression of LOXL1 in Premenopausal and Postmenopausal Women With Pelvic Organ Prolapse

Female Pelvic Medicine & Reconstructive Surgery
Nathan KowMargot S Damaser

Abstract

This study aimed to compare cellular expression of lysyl oxidase-like 1 (LOXL1), a key enzyme in elastin metabolism, of premenopausal women with pelvic organ prolapse (POP) compared with premenopausal controls without POP and postmenopausal women with POP. In addition, we examined whether variation of LOXL1 expression was dependent on biopsy site. A standardized protocol was utilized to obtain vaginal biopsies from 30 women (10 premenopausal POP, 10 postmenopausal POP, and 10 premenopausal non-POP). Expression levels of messenger RNA (mRNA) and protein of LOXL1 were determined using real-time quantitative polymerase chain reactions and enzyme-linked immunosorbant assays. Analysis was performed to determine if there were differences between group or biopsy site. Significant differences in LOXL1 mRNA expression were found between patient groups (P = 0.0033). LOXL1 mRNA expression (relative to 18S) was upregulated in the postmenopausal POP group (54.5 ± 14.7) compared with the premenopausal POP group (5.2 ± 14.7, P = 0.0034) and the premenopausal non-POP group (23 ± 18, P = 0.0359). No significant differences in LOXL1 protein expression (nanogram/milliliter per microgram total protein) were seen between groups (premenopausal POP, ...Continue Reading

References

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Citations

Jul 12, 2016·Rejuvenation Research·Léa MoulinRomain Debret
Sep 17, 2016·Female Pelvic Medicine & Reconstructive Surgery·Ali BorazjaniMargot S Damaser
Aug 11, 2017·Scientific Reports·Maryam B KhadzhievaLyubov E Salnikova
Apr 18, 2020·Menopause : the Journal of the North American Menopause Society·Ravali A ReddyFrank Z Stanczyk
Mar 27, 2021·Annals of Biomedical Engineering·Shataakshi DahalMargot S Damaser

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