PMID: 9526609Apr 4, 1998Paper

Vaginal immunization with recombinant gram-positive bacteria

American Journal of Reproductive Immunology : AJRI
Donata MedagliniG Pozzi

Abstract

Many viral and bacterial pathogens enter the body through the genital mucosa. Therefore, one of the major goals of a vaccine against sexually transmitted diseases (STDs) should be to induce an immune response in the genital mucosa capable of controlling the entry of the pathogen. Our approach for the development of vaccines against STDs is based on the use of nonpathogenic Gram-positive bacteria as live vaccine vectors. Recombinant Gram-positive bacteria expressing vaccine antigens were constructed using genetic systems developed in our laboratory. Balb/c mice and Cynomolgus monkeys were inoculated by the vaginal route and vaginal samples were collected using absorbent wicks. Colonization was evaluated by the presence of recombinant bacteria in the vaginal samples. Local and systemic immune responses were studied. We have developed genetic systems for the expression of heterologous antigens on the surface of the human commensals Streptococcus gordonii and Lactobacillus spp. Both S. gordonii and L. casei stably colonized the murine vagina after a single inoculum. Vaginal colonization of mice with recombinant strains of S. gordonii, expressing human papillomavirus (HPV) and human immunodeficiency virus (HIV) antigens, induced ant...Continue Reading

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Citations

Jun 25, 2013·Pharmaceutical Research·Muhammad Sajid Hamid AkashShuqing Chen
Feb 16, 2000·Journal of Reproductive Immunology·J W ZinckgrafL K Silbart
Aug 23, 2001·Advanced Drug Delivery Reviews·N MielcarekC Locht
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Feb 24, 2005·Clinical Obstetrics and Gynecology·Luis Alfonso Padilla-Paz
Nov 22, 2005·Expert Opinion on Drug Delivery·Lothar Steidler
Oct 24, 2006·Annals of the New York Academy of Sciences·Lothar Steidler, Pieter Rottiers
Apr 8, 2010·Expert Review of Vaccines·Vicky JespersAnna-Lena Spetz
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