Validated LC-MS/MS assay for the quantitative determination of clematichinenoside AR in rat plasma and its application to a pharmacokinetic study

Biomedical Chromatography : BMC
Dawei WangXiaodong Liu

Abstract

This study aimed to develop and validate a liquid chromatography tandem mass spectrometry method for measuring clematichinenoside AR in rat plasma. Clematichinenoside AR was extracted by solid-phase extraction and chromatographed on an XTerra MS C(8) column. Pulchinenoside B4 was used as the internal standard. Elution was achieved using an isocratic mobile phase of acetonitrile with 0.1% acetic acid (21:79, v/v) at a flow-rate of 0.2 mL/min. The detection was performed by multiple reaction monitoring mode via a negative electrospray ionization interface. Standard curves were linear, ranging from 2.5 to 500 ng/mL. The intra- and inter-day precision values were <14.0% and the accuracy was within ±13%. Extraction recovery ranged from 93.2 to 93.9%. This proposed method was successfully applied to a pharmacokinetic study on clematichinenoside AR in rats after oral administration.

References

Sep 25, 2004·Journal of Natural Products·Yoshihiro MimakiYutaka Sashida
Nov 28, 2006·Journal of Natural Products·Shepo ShiPengfei Tu
Apr 20, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Shepo ShiPengfei Tu
Jun 9, 2009·Journal of Asian Natural Products Research·Li-Fang LiuQiu-Ling Tang

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Citations

Dec 25, 2015·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Thamir M AlshammariMohamad Aljofan

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