Validated spectrofluorimetric method for the determination of carbamazepine in pharmaceutical dosage forms after reaction with 4-chloro-7--nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)

Luminescence : the Journal of Biological and Chemical Luminescence
Mohammed I WalashHanany Askar

Abstract

A sensitive and simple spectrofluorimetric method has been developed and validated for the determination of the anti-epileptic drug carbamazepine (CBZ) in its dosage forms. The method was based on a nucleophilic substitution reaction of CBZ with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in borate buffer (pH 9) to form a highly fluorescent derivative that was measured at 530 nm after excitation at 460 nm. Factors affecting the formation of the reaction product were studied and optimized, and the reaction mechanism was postulated. The fluorescence-concentration plot is rectilinear over the range of 0.6-8 µg/mL with limit of detection of 0.06 µg/mL and limit of quantitation of 0.19 µg/mL. The method was applied to the analysis of commercial tablets and the results were in good agreement with those obtained using the reference method. Validation of the analytical procedures was evaluated according to ICH guidelines.

References

Aug 11, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·S HärtterK Unger
Jul 2, 2003·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Sang Hak LeeJung Kee Suh
Jan 16, 2007·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Hanaa M SalehSoad S Abd El-Hay
Dec 7, 2007·Chemical & Pharmaceutical Bulletin·Nahed El-EnanyFathalla Belal
May 20, 2008·Journal of Pharmaceutical and Biomedical Analysis·Regina Helena Costa QueirozMaria Eugênia Costa Queiroz
Oct 17, 2008·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Armağan Onal, Olcay Sagirli
Nov 4, 2008·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Sevgi Tatar Ulu
Nov 26, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Marios MaroulisPantelis Rigas
Aug 4, 2010·Analytica Chimica Acta·Alfredina VeigaJorge Ginja Teixeira
Jan 11, 2011·Journal of Hazardous Materials·Babu Rajendran RamaswamyD G Joakim Larsson
Mar 1, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·B R LopesQ B Cass
Jan 1, 2012·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Hammam A MowafyGamal M El Maghraby
Jun 8, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Boštjan MartincTomaž Vovk
Oct 1, 2012·Journal of Pharmaceutical Analysis·Ashraf M MahmoudNiveen A Mohamed

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