Validating Alzheimer's disease micro RNAs using next-generation sequencing

Alzheimer's & Dementia : the Journal of the Alzheimer's Association
Andreas KellerEckart Meese

Abstract

Molecular biomarkers for Alzheimer's disease (AD) can support detection and improved care for patients, but novel candidates require verification. We previously reported a 12-micro RNA (miRNA) blood-based signature using next-generation sequencing (NGS) of 54 AD cases and 22 controls. We performed validation of 49 AD cases and 55 controls using NGS and also included 20 mild cognitive impairment and 90 multiple sclerosis samples to identify nonspecific markers. Thus, 103 AD cases, 77 unaffected controls, and 110 diseased controls were sequenced. Although the initial cohort came predominantly from the United States, the validation samples were collected in Germany. Five hundred eighty miRNAs were detected in the blood. In the initial cohort, we observed 203, in the validation cohort, 146 dysregulated miRNAs at a significance level of 0.05. With 68 miRNAs, the overlap was significant (P = .0003). Likewise, the area under the receiver operator characteristic curve values of the miRNAs correlated (correlation of 0.93; 95% confidence interval 0.89-0.96; P <10(-16)). MiRNAs have the potential to support AD diagnosis and patient care.

References

Sep 6, 2002·Journal of Alzheimer's Disease : JAD·Andrei TomashevskiInez Vincent
Apr 29, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xue ZhangHuaxi Xu
May 29, 2007·Nucleic Acids Research·Christina BackesHans-Peter Lenhof
Jun 26, 2007·Acta Neurologica Scandinavica·I MateoO Combarros
Jul 23, 2009·JAMA : the Journal of the American Medical Association·Niklas MattssonKaj Blennow
Jan 9, 2010·Neuroscience Letters·Yuma SonodaToshio Kawamata
Sep 6, 2011·Nature Methods·Andreas KellerEckart Meese
Sep 14, 2011·Nucleic Acids Research·Marc R FriedländerNikolaus Rajewsky
Jul 18, 2012·Archives of Neurology·James D DoeckeUNKNOWN Australian Imaging Biomarker and Lifestyle Research Group
Jul 31, 2013·Genome Biology·Petra LeidingerAndreas Keller
Sep 13, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sabrina AbsalonAnna M Krichevsky
Nov 28, 2013·Nucleic Acids Research·Ana Kozomara, Sam Griffiths-Jones
Jan 23, 2014·JAMA : the Journal of the American Medical Association·Nicolai A SchultzJulia S Johansen
Mar 13, 2014·Nature Medicine·Mark MapstoneHoward J Federoff
Jul 6, 2014·Clinical Chemistry·Benjamin MederAndreas Keller
Jan 27, 2015·Clinical Chemistry·Andreas KappelAndreas Keller
Feb 28, 2015·Gerontology·Sachli ZafariAndreas Keller
Aug 1, 2015·Nature Methods·Harsh Dweep, Norbert Gretz

❮ Previous
Next ❯

Citations

Jul 6, 2016·Molecular Diagnosis & Therapy·Christina BackesAndreas Keller
May 20, 2017·Journal of Clinical Immunology·Nizar MahlaouiAndrew Cant
Oct 17, 2017·Nucleic Acids Research·Christina BackesAndreas Keller
May 1, 2019·RNA Biology·Nicole LudwigChristina Backes
Sep 27, 2018·Current Neuropharmacology·Carmen Pena-BautistaConsuelo Chafer-Pericas
Jul 23, 2019·Critical Reviews in Clinical Laboratory Sciences·Peter D Fransquet, Joanne Ryan
Apr 25, 2018·Clinical Chemistry·Nicole LudwigAndreas Keller
Apr 3, 2019·Nucleic Acids Research·Tobias FehlmannAndreas Keller
Apr 11, 2019·International Journal of Molecular Sciences·Seongju LeeJu-Hee Kang
Nov 30, 2016·Clinical Epigenetics·Tobias FehlmannAndreas Keller
Dec 12, 2018·Neural Regeneration Research·Bridget Martinez, Philip V Peplow
May 2, 2020·Nucleic Acids Research·Fabian KernTobias Fehlmann
Sep 26, 2020·Frontiers in Molecular Neuroscience·Wei WeiHao Li
Jun 27, 2019·Molecular Neurobiology·Soraya Herrera-EspejoÁfrica Garcia-Orad
Sep 16, 2017·Nucleic Acids Research·Tobias FehlmannAndreas Keller
Oct 10, 2018·Nature Reviews. Neurology·Harald HampelKaj Blennow
Mar 2, 2019·Nucleic Acids Research·Julia AllesEckart Meese
Jan 1, 2020·BMC Bioinformatics·Jérémy AmandAndreas Keller
Jul 12, 2018·Journal of Alzheimer's Disease : JAD·Morena ZussoPietro Giusti
Nov 26, 2020·Nature Communications·Tobias FehlmannAndreas Keller
Dec 9, 2020·Nucleic Acids Research·Yongping LiAndreas Keller
Dec 10, 2019·Progress in Neurobiology·M M J van den BergJ J Briedé
Dec 15, 2020·Briefings in Bioinformatics·Georges Pierre SchmartzAndreas Keller
Apr 20, 2021·Nucleic Acids Research·Tobias FehlmannAndreas Keller

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
Kjersti TjensvollOddmund Nordgård
Archives of Neurology
James D DoeckeAustralian Imaging Biomarker and Lifestyle Research Group
© 2021 Meta ULC. All rights reserved