Validation and clinical application of a molecular method for identification of Histoplasma capsulatum in human specimens in Colombia, South America.

Clinical and Vaccine Immunology : CVI
Cesar MuñozAngel González

Abstract

The conventional means of diagnosis of histoplasmosis presents difficulties because of the delay to the time that the diagnosis is made, indicating the need for the implementation of molecular assays. We evaluated 146 clinical samples from 135 patients suspected of having histoplasmosis using a previously reported nested PCR assay for the Histoplasma capsulatum-specific 100-kDa protein (the Hc100 PCR). In order to determine the specificity of this molecular test, we also used samples from healthy individuals (n = 20), patients suspected of having respiratory disease with negative fungal cultures (n = 29), and patients with other proven infections (n = 60). Additionally, a sizable collection of DNA from cultures of H. capsulatum and other medically relevant pathogens was studied. A panfungal PCR assay that amplified the internal transcribed spacer 2 region was also used to identify all fungal DNAs. All PCR-amplified products were sequenced. Of the 146 clinical samples, 67 (45.9%) were positive by culture and PCR, while 9 samples negative by culture were positive by PCR. All the sequences corresponding to the 76 amplified products presented > or =98% identity with H. capsulatum. The Hc100 PCR exhibited a sensitivity of 100% and s...Continue Reading

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Citations

Dec 14, 2011·Epidemiology and Infection·A E González-GonzálezM L Taylor
Dec 23, 2011·Journal of Clinical Microbiology·María Guadalupe Frías De LeónMaría Del Rocío Reyes-Montes
Mar 20, 2013·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Antonio Ernesto González-GonzálezMaria Lucia Taylor
May 27, 2014·Current Tropical Medicine Reports·Antoine A AdenisPierre Couppié
Nov 21, 2013·Revista iberoamericana de micología·Beatriz L Gómez
May 27, 2014·Clinics in Laboratory Medicine·Kevin C Hazen
Apr 16, 2013·Revista iberoamericana de micología·María José BuitragoManuel Cuenca-Estrella
Nov 1, 2011·Revista iberoamericana de micología·Antonio Ernesto González-GonzálezEverardo Curiel-Quesada
Oct 12, 2016·Folia Microbiologica·María Guadalupe Frías-De-LeónMaría Del Rocío Reyes-Montes
Jul 1, 2015·The American Journal of Tropical Medicine and Hygiene·Diego H CaceresÁngel González
Jan 31, 2014·Journal of Clinical Microbiology·Christina M ScheelAnastasia P Litvintseva
Aug 27, 2019·PLoS Neglected Tropical Diseases·Matheus da Silva ZattiRaquel Cordeiro Theodoro
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Jan 1, 2014·Current Tropical Medicine Reports·Christina M Scheel, Beatriz L Gómez
Feb 16, 2021·Applied Microbiology and Biotechnology·María Agustina ToscaniniMaría Luján Cuestas
May 1, 2021·Journal of Fungi·Priscila Marques de MacedoRodrigo Almeida-Paes
May 22, 2021·Infectious Disease Clinics of North America·Ana Belén Araúz, Padmasayee Papineni

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