PMID: 12755433May 21, 2003Paper

Validation and clinical utility of a novel immunoradiometric assay exclusively for biologically active whole parathyroid hormone in the horse

Equine Veterinary Journal
Jose C EstepaEscolastico Aguilera-Tejero

Abstract

Parathyroid hormone (PTH) plays a critical role in the regulation of mineral metabolism in mammals. Until recently, the standard method for PTH measurement has been the 2nd generation intact-PTH (I-PTH) assay. Current evidence indicates that the I-PTH assay binds to the PTH molecule and to an inactive N-terminally truncated PTH fragment that tends to accumulate in the blood of uraemic patients. Therefore, a new 3rd generation PTH assay that detects only the whole PTH molecule (W-PTH; cyclase-activating PTH [CAP]) has been developed. To validate this more specific W-PTH assay for measurement of equine PTH and evaluate its clinical utility. W-PTH and I-PTH were measured in plasma samples from normal horses (adults and foals) and horses with nutritional secondary hyperparathyroidism (N2HPT) and with chronic renal failure (CRF). Replicate measurements and dilutional paralellism were used for assay validation. Changes in blood ionized calcium were induced by EDTA and CaCl2 administration. Performance of the W-PTH assay (accuracy, sensitivity, specificity and ability to detect changes in PTH in response to changes in calcium) was similar to that of the I-PTH assay. Surprisingly, the relative W-PTH concentration in normal horses and f...Continue Reading

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Aug 24, 2001·Journal of the American Veterinary Medical Association·E Aguilera-TejeroM Rodríguez

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Citations

Jan 13, 2006·Veterinary Pathology·J C EstepaJ Perez
Mar 28, 2012·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Gwen JankowskiDennis Schmitt
Jan 28, 2010·Journal of Veterinary Internal Medicine·I DurieP Deprez
Apr 14, 2005·Domestic Animal Endocrinology·Sonia BasEscolástico Aguilera-Tejero

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