Validation and comparative analysis of a multiplexed assay for the simultaneous quantitative measurement of Th1/Th2 cytokines in human serum and human peripheral blood mononuclear cell culture supernatants

Journal of Immunological Methods
Uma PrabhakarBruce E Miller

Abstract

There is increasing evidence suggesting a relationship between cytokine levels and disease pathogenesis, which has led to interest in analyzing multiple cytokines in biological fluids and culture supernatants for various research and clinical studies. The introduction of methodologies allowing simultaneous measurement of interrelated biomarkers/cytokines has further revolutionized this process. In contrast to tissue culture supernatant, the measurement of cytokines in serum has proven to be difficult to characterize in multiplexed formats because of the presence of large dynamic concentration ranges of proteins and other interfering factors that are present in this matrix. In the present study, we have used the microsphere-based multiplex method to simultaneously quantitate and compare six analytes, encompassing a representation of the Th1/Th2 cytokine panel (interleukin (IL)-2, IL-4, IL-5, interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), and IL-10), in both serum and culture supernatants from peripheral blood mononuclear cells (PBMCs). A detailed validation procedure for these determinations is described along with a comparative analysis of the performance of the multiplexed assay in serum and culture sup...Continue Reading

References

Jun 1, 1990·Immunology Today·C H JuneC B Thompson
Jun 1, 1993·The International Journal of Biochemistry·F LicastroM C Morini
Feb 14, 1998·Transfusion Medicine Reviews·J L Ferrara, W Krenger
Sep 15, 1999·Journal of Immunological Methods·R T Carson, D A Vignali
Apr 20, 2000·The Journal of Clinical Investigation·J L Ferrara
Apr 25, 2000·Annual Review of Medicine·K A Papadakis, S R Targan
Sep 15, 2000·Journal of Immunological Methods·D A Vignali
Jan 17, 2002·Journal of Immunological Methods·Uma PrabhakarHugh M Davis
Nov 9, 2002·Experimental Hematology·Kathryn L Kellar, Marie A Iannone
Dec 4, 2003·Journal of Internal Medicine·K KatcharJ Grunewald

❮ Previous
Next ❯

Citations

Dec 12, 2012·The British Journal of Nutrition·Béatrice GleizePatrick Borel
Nov 2, 2011·Journal of the American Chemical Society·Matthew S Luchansky, Ryan C Bailey
Aug 20, 2010·Nature Reviews. Cancer·Donal J BrennanWilliam M Gallagher
Oct 26, 2005·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Hua TanLawrence M Blatt
Sep 6, 2008·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Sean X LengGeorge A Kuchel
Jul 27, 2010·Current Opinion in Clinical Nutrition and Metabolic Care·Xin ZhouGeorge A Kuchel
Apr 20, 2014·Journal of Pharmaceutical and Biomedical Analysis·Kati HuttunenMaija-Riitta Hirvonen
May 20, 2014·Journal of Periodontology·Getulio Nogueira-FilhoChristopher A McCulloch
Jan 12, 2012·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·Burel R GoodinLynanne McGuire
May 16, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Andrea BeyerleTobias Stoeger
Nov 26, 2008·Veterinary Immunology and Immunopathology·Bettina Wagner, Heather Freer
Sep 11, 2007·The Journal of Allergy and Clinical Immunology·Eva-Maria KingMartin D Chapman
Apr 7, 2007·Journal of Reproductive Immunology·Toshiyuki YasuiMinoru Irahara
May 22, 2007·Veterinary Immunology and Immunopathology·Johanna DernfalkAnders Johannisson
Dec 14, 2006·Maturitas·Toshiyuki YasuiMinoru Irahara
Jan 14, 2012·Transplant International : Official Journal of the European Society for Organ Transplantation·Cecilia M ShingJeff S Coombes
Aug 22, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Yu ShangTobias Stoeger
Aug 25, 2012·Journal of Immunological Methods·Chahrazed BelabaniUNKNOWN Canadian Pediatric Demyelinating Disease Network
Apr 30, 2015·PLoS Computational Biology·Kevin ThurleyThomas Höfer
Oct 8, 2005·Expert Review of Proteomics·Josef G HeuerBrian T Edmonds
Jul 13, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Valérie RodriguesIsabelle Chantal
May 14, 2011·Journal of Biophotonics·Valentina Donzella, Francesco Crea
Feb 17, 2018·Scientific Reports·Elisabeth KarstenBenjamin R Herbert
Aug 26, 2009·Scandinavian Journal of Immunology·E AktasG Deniz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.