Validation and Cross-Reactivity Data for Fentanyl Analogs With the Immunalysis Fentanyl ELISA

Journal of Analytical Toxicology
Davide GuerrieriHenrik Gréen

Abstract

Every year new fentanyl analog compounds, or fentanyls, appear on the drug scene. Development of immunoassays dedicated for screening individual molecules is challenging due to the short-lived presence of these compounds on the recreational drug market. Therefore, we investigated the detecting capabilities of the immunalysis fentanyl direct enzyme-linked immunosorbent assay (ELISA) kit against fentanyl in whole blood, and determined the cross-reactivity of nine fentanyl analogs (2-fluorofentanyl, acetylfentanyl, acrylfentanyl, carfentanil, cyclopropylfentanyl, tetrahydrofuranylfentanyl, furanylfentanyl, ocfentanil, valerylfentanyl) to confirm its validity for the general screening of fentanyls. Immunalysis ELISA assay was used to test whole blood samples fortified with fentanyl on a TECAN Freedom EVOlyzer platform, according to manufacturer specifications. The kit successfully was validated for fentanyl screening with a cutoff set at 0.5 ng/mL, and all tested analogs, with the exclusion of carfentanil, were detected. The lowest cross-reactivity with the kit was obtained with furanylfentanyl (20% ± 1, 95% confidence intervals (CI)) and 4-fluoroisobutyrfentanyl (25% ± 1, 95% CI), while the highest was recorded using acetylfentany...Continue Reading

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Citations

Mar 29, 2019·Critical Reviews in Toxicology·Annelies CannaertChristophe P Stove
Jul 17, 2020·The Journal of Applied Laboratory Medicine·Melissa M BudelierChristopher W Farnsworth
Aug 21, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xianlu LeiChuanlai Xu
Nov 25, 2020·Therapeutic Drug Monitoring·Gwendolyn A McMillinYifei K Yang
Jan 2, 2021·Forensic Science International : Synergy·Nicole S Jones, Jeffrey H Comparin
Jan 2, 2021·Forensic Science International : Synergy·Wing-Sum ChanBobbie Kwok-Keung Cheung
Feb 14, 2021·Journal of Analytical Toxicology·Rebekah E WhartonRudolph C Johnson
Mar 23, 2021·Harm Reduction Journal·Tracy-Lynn E LockwoodMarya Lieberman
Nov 16, 2021·Journal of Medical Toxicology : Official Journal of the American College of Medical Toxicology·Andrew StolbachKen Kulig

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