Abstract
Every year new fentanyl analog compounds, or fentanyls, appear on the drug scene. Development of immunoassays dedicated for screening individual molecules is challenging due to the short-lived presence of these compounds on the recreational drug market. Therefore, we investigated the detecting capabilities of the immunalysis fentanyl direct enzyme-linked immunosorbent assay (ELISA) kit against fentanyl in whole blood, and determined the cross-reactivity of nine fentanyl analogs (2-fluorofentanyl, acetylfentanyl, acrylfentanyl, carfentanil, cyclopropylfentanyl, tetrahydrofuranylfentanyl, furanylfentanyl, ocfentanil, valerylfentanyl) to confirm its validity for the general screening of fentanyls. Immunalysis ELISA assay was used to test whole blood samples fortified with fentanyl on a TECAN Freedom EVOlyzer platform, according to manufacturer specifications. The kit successfully was validated for fentanyl screening with a cutoff set at 0.5 ng/mL, and all tested analogs, with the exclusion of carfentanil, were detected. The lowest cross-reactivity with the kit was obtained with furanylfentanyl (20% ± 1, 95% confidence intervals (CI)) and 4-fluoroisobutyrfentanyl (25% ± 1, 95% CI), while the highest was recorded using acetylfentany...Continue Reading
References
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A P FeinbergS H Snyder
Aug 10, 2001·Journal of Pain and Symptom Management·J PereiraE Bruera
Jul 11, 2006·Bioorganic & Medicinal Chemistry Letters·Ravil R PetrovVictor J Hruby
Mar 12, 2010·Journal of Medicinal Chemistry·Grazyna WeltrowskaPeter W Schiller
Apr 3, 2010·Clinical Chemistry·David M SchwopeMarilyn A Huestis
Mar 19, 2014·Future Medicinal Chemistry·Ruben S Vardanyan, Victor J Hruby
Apr 29, 2015·Journal of Analytical Toxicology·Iain M McIntyreJonathan R Lucas
Jun 8, 2017·Forensic Science International : Synergy·Davide GuerrieriRobert Kronstrand
Citations
Mar 29, 2019·Critical Reviews in Toxicology·Annelies CannaertChristophe P Stove
Aug 20, 2019·Journal of Analytical Toxicology·Kristin W KahlLisa J Reidy
Feb 28, 2020·Journal of Applied Toxicology : JAT·Piotr AdamowiczAnna Mikolajczyk
Jul 17, 2020·The Journal of Applied Laboratory Medicine·Melissa M BudelierChristopher W Farnsworth
Feb 28, 2020·Journal of Analytical Toxicology·Jakob WallgrenPeter Konradsson
Aug 21, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xianlu LeiChuanlai Xu
Nov 25, 2020·Therapeutic Drug Monitoring·Gwendolyn A McMillinYifei K Yang
Jan 2, 2021·Forensic Science International : Synergy·Nicole S Jones, Jeffrey H Comparin
Jan 2, 2021·Forensic Science International : Synergy·Wing-Sum ChanBobbie Kwok-Keung Cheung
Feb 14, 2021·Journal of Analytical Toxicology·Rebekah E WhartonRudolph C Johnson
Mar 2, 2021·Journal of Analytical Toxicology·Lu LiYurong Zhang
Mar 23, 2021·Harm Reduction Journal·Tracy-Lynn E LockwoodMarya Lieberman
Nov 16, 2021·Journal of Medical Toxicology : Official Journal of the American College of Medical Toxicology·Andrew StolbachKen Kulig
Nov 19, 2021·Journal of Analytical Toxicology·Rebecca MastrovitoBarry Logan