Validation and inter-examiner agreement of mutans streptococci levels in plaque and saliva of 10-year-old children using simple chair-side tests

Acta Odontologica Scandinavica
Sára KarjalainenKaisu Pienihäkkinen

Abstract

Since there are few validation studies of chair-side tests of oral mutans streptococci, we compared a commercially available strip test with the conventional laboratory assay. Two plaque samples obtained from the mesial surfaces of the upper right and lower left permanent molars of sixty-five 10-year-old children (boys = 38, girls = 27) were cultured and incubated using chair-side site strip tests (Dentocult SM, Orion Diagnostica). Two plaque sampling tools, namely dental floss and micro-brush, were compared, and inter-examiner agreement between recordings of three examiners was assessed. Paraffin-stimulated saliva was then collected for laboratory and chair-side assays. The plaque and saliva chair-side tests correlated well with each other (Spearman rho, r = 0.72) and with the laboratory method, showing coefficients of 0.76 and 0.80 for saliva and plaque, respectively. Compared to the laboratory method, the sensitivity (Sn), specificity (Sp), accuracy (A), and kappa (K) values of the salivary and plaque chair-side tests were 0.63, 0.75 (Sn), 0.93, 0.90 (Sp), 0.82, 0.85 (A), and 0.58, 0.66 (K), respectively. Agreement between the two plaque sampling techniques was good (0.91). Inter-examiner agreement of plaque scores ranged be...Continue Reading

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Citations

Jun 7, 2008·European Archives of Paediatric Dentistry : Official Journal of the European Academy of Paediatric Dentistry·I WennhallS Twetman
Mar 28, 2012·European Archives of Paediatric Dentistry : Official Journal of the European Academy of Paediatric Dentistry·J OlakS Karjalainen
Mar 26, 2014·Clinical Oral Investigations·Isabelle LalemanWim Teughels
Jul 22, 2014·Special Care in Dentistry : Official Publication of the American Association of Hospital Dentists, the Academy of Dentistry for the Handicapped, and the American Society for Geriatric Dentistry·Juhea ChangHae-Young Kim
Sep 4, 2014·European Journal of Oral Sciences·Suchitra NelsonPeter Milgrom
Apr 14, 2010·International Journal of Paediatric Dentistry·Pia MeurmanPentti Alanen
Aug 21, 2013·Journal of Dentistry·Mohamed ElSalhyEino Honkala
Oct 24, 2012·Acta Odontologica Scandinavica·Jung-Hyun LeeJuhea Chang
Dec 2, 2009·Journal of Dental Research·Y NakaiM Takimura

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