Validation of a CD30 Enzyme-Linked Immunosorbant Assay for the Rapid Detection of Breast Implant-Associated Anaplastic Large Cell Lymphoma

Aesthetic Surgery Journal
Summer E HansonMark W Clemens

Abstract

Breast implant-associated anaplastic large cell lymphoma (BIA-ALCL) is an uncommon type of non-Hodgkin lymphoma occurring in the fluid or capsule adjacent to textured breast implants. Diagnosis of BIA-ALCL of symptomatic patients requires demonstration of large anaplastic cells with uniform expression of CD30 protein on immunohistochemistry. The authors investigated a novel, rapid, office-based, and economic in-situ enzyme-linked immunosorbent assay (ELISA) for screening BIA-ALCL patients. A commercially available in-situ ELISA was standardized and validated for patients with confirmed BIA-ALCL diagnosis with clinical isolates. A panel of 9 pathologically confirmed BIA-ALCL patients was screened by serum, plasma, and periprosthetic effusion specimens and compared against serum, plasma, and nonneoplastic delayed seromas in 7 control patients. Statistical analysis demonstrated assay consistency and reliability. All BIA-ALCL effusions demonstrated CD30 ELISA detection at full and all serial concentrations. BIA-ALCL serum specimens and all control specimens were negative at full concentration and serial dilutions (1:100, 1:250, 1:500, and 1:1000). BIA-ALCL plasma specimens were weakly positive at full concentration and revealed no ...Continue Reading

References

Nov 6, 2008·JAMA : the Journal of the American Medical Association·Daphne de JongFlora E van Leeuwen
Jul 17, 2009·Clinical and Vaccine Immunology : CVI·Igor PavlovJulio C Delgado
Sep 13, 2012·Leukemia & Lymphoma·Ashkan Emadi, Judith E Karp
Dec 11, 2013·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Roberto N MirandaL Jeffrey Medeiros
Sep 27, 2015·Clinics in Plastic Surgery·Mark W Clemens, Roberto N Miranda
Dec 3, 2015·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Mark W ClemensRoberto N Miranda
Jul 9, 2016·British Journal of Hospital Medicine·Karishma Shah, Panagiotis Maghsoudlou
Feb 6, 2017·Plastic and Reconstructive Surgery·Erin L DorenMark W Clemens
Apr 13, 2017·Aesthetic Surgery Journal·Marshall E KadinCaroline A Glicksman
Mar 30, 2018·Plastic and Reconstructive Surgery·Mark W ClemensRoberto N Miranda

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