Validation of a confirmatory method for the determination of sulphonamides in muscle according to the European Union regulation 2002/657/EC

Journal of Chromatography. a
Ivan PecorelliRoberta Galarini

Abstract

A simple multiresidue method is described for assaying 10 sulphonamides (SAs) (sulfadiazine, sulfathiazole, sulfapyridine, sulfamerazine, sulfamethazine, sulfamonomethoxine, sulfachlorpyridazine, sulfamethoxazole, sulfaquinoxaline and sulfadimethoxine) in muscle samples. Samples were prepared by homogenizing the tissue, extracting with ethyl acetate and cleaning up with a cation-exchange solid-phase extraction (SPE) column. The detection of analytes was achieved by HPLC-diode array detection (DAD) at 270 nm. The procedure was validated according to the European Union regulation 2002/657/EC determining specificity, decision limit, detection capability, trueness and precision. The results of validation process demonstrate that the method is suitable for application in European Union statutory veterinary drug residue surveillance programmes.

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Related Concepts

Metazoa
Calibration
High Pressure Liquid Chromatography Procedure
European Atomic Energy Community
Muscle
Statistical Sensitivity
Spectrophotometry, Ultraviolet
Sulfonamides
Reproducibility of Results

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