Validation of a confirmatory method for the determination of sulphonamides in muscle according to the European Union regulation 2002/657/EC

Journal of Chromatography. a
Ivan PecorelliRoberta Galarini


A simple multiresidue method is described for assaying 10 sulphonamides (SAs) (sulfadiazine, sulfathiazole, sulfapyridine, sulfamerazine, sulfamethazine, sulfamonomethoxine, sulfachlorpyridazine, sulfamethoxazole, sulfaquinoxaline and sulfadimethoxine) in muscle samples. Samples were prepared by homogenizing the tissue, extracting with ethyl acetate and cleaning up with a cation-exchange solid-phase extraction (SPE) column. The detection of analytes was achieved by HPLC-diode array detection (DAD) at 270 nm. The procedure was validated according to the European Union regulation 2002/657/EC determining specificity, decision limit, detection capability, trueness and precision. The results of validation process demonstrate that the method is suitable for application in European Union statutory veterinary drug residue surveillance programmes.


Mar 1, 1990·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·N A LittlefieldD W Gaylor
Sep 27, 1985·Journal of Chromatography·N Haagsma, C van de Water


Mar 15, 2006·Analytical and Bioanalytical Chemistry·M Gratacós-CubarsíJ A García-Regueiro
Mar 21, 2006·Food Additives and Contaminants·S WangI Kennedy
Apr 29, 2006·Food Additives and Contaminants·Mervi Rokka, Kimmo Peltonen
Jun 18, 2009·Analytical and Bioanalytical Chemistry·F Cañada-CañadaA Espinosa-Mansilla
May 17, 2014·Analytica Chimica Acta·M Isabel LópezM Pilar Callao
Jul 15, 2009·Journal of Chromatography. a·Petra Gowik
May 19, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·C J KowalskiM Osypiuk
Jan 1, 2008·Meat Science·Milagro Reig, Fidel Toldrá
Oct 20, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Ahmad Soleymanpour, Seyyed Ahmad Rezvani
Jul 22, 2010·Journal of Veterinary Pharmacology and Therapeutics·B I Łebkowska-Wieruszewska, C J Kowalski
Mar 17, 2009·Analytica Chimica Acta·A F Forti, G Scortichini
Mar 23, 2013·Acta Veterinaria Scandinavica·Taneli TirkkonenJaakko Pakarinen
Jul 18, 2009·Analytical and Bioanalytical Chemistry·Sara Bogialli, Antonio Di Corcia
Jul 29, 2009·Analytical and Bioanalytical Chemistry·R CompanyóM D Prat
Sep 19, 2009·American Journal of Industrial Medicine·Alberto ScarselliSergio Iavicoli
Jan 9, 2010·Risk Analysis : an Official Publication of the Society for Risk Analysis·Hajo RijgersbergMieke Uyttendaele
Mar 11, 2010·Analytical and Bioanalytical Chemistry·J Raich-MontiuM Granados
Mar 7, 2006·Food Additives and Contaminants·A-C MartelM Aubert
Oct 25, 2007·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Martin DanaherChristopher T Elliott
Dec 15, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sebastian WolfTorsten Frosch
Mar 13, 2019·Nature Ecology & Evolution·Li ZhangManyuan Long

Related Concepts

High Pressure Liquid Chromatography Procedure
European Atomic Energy Community
Statistical Sensitivity
Spectrophotometry, Ultraviolet
Reproducibility of Results

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.