Validation of a Dictyocaulus viviparus MSP-ELISA and cut-off adjustment in a one-year longitudinal field study in dairy cattle herds

Veterinary Parasitology
Anne-Marie SchunnChristina Strube

Abstract

A one-year field study analysing lungworm seropositivity by use of the MSP-ELISA was performed (1) to investigate the antibody dynamics in individual milk samples following field (re-)infections of dairy cows with the bovine lungworm Dictyocaulus viviparus, (2) to investigate the correlation between individual and bulk tank milk (BTM) antibody titres and (3) to review the current individual as well as BTM cut-off value, which was extrapolated from dilution experiments (Fiedor et al., 2009). Over a one-year period individual and BTM samples were collected monthly on 15 dairy farms. Following a critical review of previous cut-off values, individual and BTM samples were subjected to different cut-off thresholds. Following Receiver-Operating-Characteristics (ROC) analysis, individual milk samples were assessed with the cut-off value 0.573, previously shown to be associated with each 100% sensitivity and specificity. In addition, the present study enabled BTM cut-off adjustment based on field data. To ensure reliable detection of herds with an in-herd prevalence of ≥20% the BTM cut-off was lowered from 0.493 to 0.410, corresponding to 100% sensitivity and 97.32% specificity. Regression analysis showed that the percentage of seroposi...Continue Reading

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Jun 1, 1997·Veterinary Parasitology·J B CornelissenF J van Milligen
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Oct 6, 2009·Veterinary Parasitology·Christiane FiedorThomas Schnieder
Sep 16, 2011·Preventive Veterinary Medicine·Anne-Marie KlewerChristina Strube
Nov 8, 2011·The Veterinary Record·Wendela Wapenaar

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Citations

Jan 12, 2013·Parasitology Research·Min-Jun XuXing-Quan Zhu
Nov 1, 2015·Veterinary Parasitology·Yris BloemhoffRiona Sayers
Jul 30, 2016·Micron : the International Research and Review Journal for Microscopy·Vladimir V YushinWim Bert
Aug 5, 2020·Parasitology Research·Simone MorelliDonato Traversa
Mar 23, 2021·Veterinary Parasitology·M VanheckeE Claerebout
Oct 22, 2019·Veterinary Parasitology: X·Catherine McCarthyJan van Dijk

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