Abstract
A one-year field study analysing lungworm seropositivity by use of the MSP-ELISA was performed (1) to investigate the antibody dynamics in individual milk samples following field (re-)infections of dairy cows with the bovine lungworm Dictyocaulus viviparus, (2) to investigate the correlation between individual and bulk tank milk (BTM) antibody titres and (3) to review the current individual as well as BTM cut-off value, which was extrapolated from dilution experiments (Fiedor et al., 2009). Over a one-year period individual and BTM samples were collected monthly on 15 dairy farms. Following a critical review of previous cut-off values, individual and BTM samples were subjected to different cut-off thresholds. Following Receiver-Operating-Characteristics (ROC) analysis, individual milk samples were assessed with the cut-off value 0.573, previously shown to be associated with each 100% sensitivity and specificity. In addition, the present study enabled BTM cut-off adjustment based on field data. To ensure reliable detection of herds with an in-herd prevalence of ≥20% the BTM cut-off was lowered from 0.493 to 0.410, corresponding to 100% sensitivity and 97.32% specificity. Regression analysis showed that the percentage of seroposi...Continue Reading
References
Jul 1, 1991·Veterinary Parasitology·W A de Leeuw, J B Cornelissen
Oct 19, 1991·The Veterinary Record·D A Wassall
Jun 1, 1994·Veterinary Parasitology·H W SaatkampJ Verhoeff
May 1, 1993·Veterinary Parasitology·T SchniederA M Tenter
May 1, 1993·Veterinary Parasitology·A M TenterT Schnieder
Jun 1, 1997·Veterinary Parasitology·J B CornelissenF J van Milligen
Jun 1, 2000·Veterinary Parasitology·J AgneessensJ Vercruysse
Jan 30, 2002·Parasitology·M EyskerH W Ploeger
Mar 18, 2006·Veterinary Parasitology·Johan Höglund
Dec 25, 2007·Veterinary Parasitology·Christian von HoltumGeorg von Samson-Himmelstjerna
Oct 6, 2009·Veterinary Parasitology·Christiane FiedorThomas Schnieder
May 15, 2010·Veterinary Parasitology·Johan HöglundSofia Sollenberg
Sep 16, 2011·Preventive Veterinary Medicine·Anne-Marie KlewerChristina Strube
Sep 16, 2011·Veterinary Parasitology·H W PloegerM Holzhauer
Nov 8, 2011·The Veterinary Record·Wendela Wapenaar
Citations
Sep 17, 2013·PloS One·Anne-Marie SchunnChristina Strube
Jan 12, 2013·Parasitology Research·Min-Jun XuXing-Quan Zhu
Feb 10, 2016·Scientific Reports·Samantha N McNultyMakedonka Mitreva
Nov 1, 2015·Veterinary Parasitology·Yris BloemhoffRiona Sayers
Feb 25, 2015·Veterinary Parasitology·Yris BloemhoffRíona Sayers
Nov 6, 2013·Veterinary Parasitology·H W PloegerE Van Engelen
Jul 30, 2016·Micron : the International Research and Review Journal for Microscopy·Vladimir V YushinWim Bert
Jan 10, 2018·Parasites & Vectors·Katharina MayChristina Strube
Aug 5, 2020·Parasitology Research·Simone MorelliDonato Traversa
Mar 23, 2021·Veterinary Parasitology·M VanheckeE Claerebout
Oct 22, 2019·Veterinary Parasitology: X·Catherine McCarthyJan van Dijk
Aug 8, 2021·Animals : an Open Access Journal From MDPI·Andrea SpringerChristina Strube