DNA methylation is the best known epigenetic mark. Cancer and other pathologies show an altered DNA methylome. However, delivering complete DNA methylation maps is compromised by the price and labor-intensive interpretation of single nucleotide methods. Following the success of the HumanMethylation450 BeadChip (Infinium) methylation microarray (450K), we report the technical and biological validation of the newly developed MethylationEPIC BeadChip (Infinium) microarray that covers over 850,000 CpG methylation sites (850K). The 850K microarray contains >90% of the 450K sites, but adds 333,265 CpGs located in enhancer regions identified by the ENCODE and FANTOM5 projects. The 850K array demonstrates high reproducibility at the 450K CpG sites, is consistent among technical replicates, is reliable in the matched study of fresh frozen versus formalin-fixed paraffin-embeded samples and is also useful for 5-hydroxymethylcytosine. These results highlight the value of the MethylationEPIC BeadChip as a useful tool for the analysis of the DNA methylation profile of the human genome.
Regions of focal DNA hypermethylation and long-range hypomethylation in colorectal cancer coincide with nuclear lamina-associated domains
The human imprintome: regulatory mechanisms, methods of ascertainment, and roles in disease susceptibility
Genome-wide parent-of-origin DNA methylation analysis reveals the intricacies of human imprinting and suggests a germline methylation-independent mechanism of establishment
Validation of DNA methylation profiling in formalin-fixed paraffin-embedded samples using the Infinium HumanMethylation450 Microarray
Accurate measurement of 5-methylcytosine and 5-hydroxymethylcytosine in human cerebellum DNA by oxidative bisulfite on an array (OxBS-array)
Bivalent Regions of Cytosine Methylation and H3K27 Acetylation Suggest an Active Role for DNA Methylation at Enhancers
Defining, distinguishing and detecting the contribution of heterogeneous methylation to cancer heterogeneity
Epigenetic dysregulation of brainstem nuclei in the pathogenesis of Alzheimer's disease: looking in the correct place at the right time?
Validation of the MethylationEPIC BeadChip for fresh-frozen and formalin-fixed paraffin-embedded tumours
Profiling DNA methylation differences between inbred mouse strains on the Illumina Human Infinium MethylationEPIC microarray
Epigenome-wide analysis reveals specific DNA hypermethylation of T cells during human hematopoietic differentiation
ASB1 differential methylation in ischaemic cardiomyopathy: relationship with left ventricular performance in end-stage heart failure patients
The correlation of methylation levels measured using Illumina 450K and EPIC BeadChips in blood samples
MACON: a web tool for computing DNA methylation data obtained by the Illumina Infinium Human DNA methylation BeadArray
Characterization of parent-of-origin methylation using the Illumina Infinium MethylationEPIC array platform
Identification of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome-associated DNA methylation patterns
Small-Magnitude Effect Sizes in Epigenetic End Points are Important in Children's Environmental Health Studies: The Children's Environmental Health and Disease Prevention Research Center's Epigenetics Working Group
Advances in the classification of pediatric brain tumors through DNA methylation profiling: From research tool to frontline diagnostic
Cell type-specific DNA methylation in neonatal cord tissue and cord blood: a 850K-reference panel and comparison of cell types
Variation in DNA methylation of human blood over a 1-year period using the Illumina MethylationEPIC array
The Intergenerational Impact of Genetic and Psychological Factors on Blood Pressure (InterGEN) Study: Design and Methods for Complex DNA Analysis
Intratumor DNA methylation heterogeneity in glioblastoma: implications for DNA methylation-based classification
Global DNA methylation of peripheral blood leukocytes from dogs bearing multicentric non-Hodgkin lymphomas and healthy dogs: A comparative study
DNA methylation signal has a major role in the response of human breast cancer cells to the microenvironment
Variability in DNA methylation at the serotonin transporter gene promoter: epigenetic mechanism or cell-type artifact?
Genome-wide profiling of DNA methylome and transcriptome in peripheral blood monocytes for major depression: A Monozygotic Discordant Twin Study
Novel age-associated DNA methylation changes and epigenetic age acceleration in middle-aged African Americans and whites
The impact of proinflammatory cytokines on the β-cell regulatory landscape provides insights into the genetics of type 1 diabetes
Genome-wide characterization of 5-hydoxymethylcytosine in melanoma reveals major differences with nevus
Epigenetic Conservation Is a Beacon of Function: An Analysis Using Methcon5 Software for Studying Gene Methylation.
Antipsychotic Medications and DNA Methylation in Schizophrenia and Bipolar Disorder: A Systematic Review
Race-specific alterations in DNA methylation among middle-aged African Americans and Whites with metabolic syndrome
Deep analysis of acquired resistance to FGFR1 inhibitor identifies MET and AKT activation and an expansion of AKT1 mutant cells
The DNA methylation landscape of human cancer organoids available at the American type culture collection.
Characterization of the ZFX family of transcription factors that bind downstream of the start site of CpG island promoters
Age prediction of children and adolescents aged 6-17 years: an epigenome-wide analysis of DNA methylation
DNA methylation-based age prediction and telomere length in white blood cells and cumulus cells of infertile women with normal or poor response to ovarian stimulation
DNA methylation in genes of longevity-regulating pathways: association with obesity and metabolic complications
A comparison of reference-based algorithms for correcting cell-type heterogeneity in Epigenome-Wide Association Studies
High-specificity bioinformatics framework for epigenomic profiling of discordant twins reveals specific and shared markers for ACPA and ACPA-positive rheumatoid arthritis
Clinical review of 24-35 year olds conceived with and without in vitro fertilization: study protocol
Genome-wide identification of inter-individually variable DNA methylation sites improves the efficacy of epigenetic association studies
DNA methylation profiling of acute chorioamnionitis-associated placentas and fetal membranes: insights into epigenetic variation in spontaneous preterm births
Systematic evaluation of DNA methylation age estimation with common preprocessing methods and the Infinium MethylationEPIC BeadChip array
Methylation at cg05575921 of a smoking-related gene (AHRR) in non-smoking Taiwanese adults residing in areas with different PM2.5 concentrations
MiR-1253 exerts tumor-suppressive effects in medulloblastoma via inhibition of CDK6 and CD276 (B7-H3)
Genome-wide identification of differentially methylated promoters and enhancers associated with response to anti-PD-1 therapy in non-small cell lung cancer
DNA Methylation Profiling Reveals Prognostically Significant Groups in Pediatric Adrenocortical Tumors: A Report From the International Pediatric Adrenocortical Tumor Registry
Placental Epigenome-Wide Association Study Identified Loci Associated with Childhood Adiposity at 3 Years of Age
Contrasting the effects of intra-uterine smoking and one-carbon micronutrient exposures on offspring DNA methylation
A new approach to epigenome-wide discovery of non-invasive methylation biomarkers for colorectal cancer screening in circulating cell-free DNA using pooled samples
Epigenetic changes in preterm birth placenta suggest a role for ADAMTS genes in spontaneous preterm birth
Association of a placental Interleukin-6 genetic variant (rs1800796) with DNA methylation, gene expression and risk of acute chorioamnionitis
Genome-wide DNA methylation analysis in obese women predicts an epigenetic signature for future endometrial cancer
Best practices in DNA methylation: lessons from inflammatory bowel disease, psoriasis and ankylosing spondylitis
Rationale, Study Design, and Cohort Characteristics for the Markers for Environmental Exposures (MEE) Study
Full-term low birth weight infants have differentially hypermethylated DNA related to immune system and organ growth: a comparison with full-term normal birth weight infants
MethCORR modelling of methylomes from formalin-fixed paraffin-embedded tissue enables characterization and prognostication of colorectal cancer
Genome-wide DNA methylation signatures to predict pathologic complete response from combined neoadjuvant chemotherapy with bevacizumab in breast cancer
Arsenic Disulfide Promoted Hypomethylation by Increasing DNA Methyltransferases Expression in Myelodysplastic Syndrome
A cell-type deconvolution meta-analysis of whole blood EWAS reveals lineage-specific smoking-associated DNA methylation changes
Epigenetic markers associated with metformin response and intolerance in drug-naïve patients with type 2 diabetes.
SPlinted Ligation Adapter Tagging (SPLAT), a novel library preparation method for whole genome bisulphite sequencing
Array probe density and pathobiological relevant CpG calling bias in human disease and physiological DNA methylation profiling
Forty-five patient-derived xenografts capture the clinical and biological heterogeneity of Wilms tumor
Epigenetic regulation of the lineage specificity of primary human dermal lymphatic and blood vascular endothelial cells
Sperm epigenetics in the study of male fertility, offspring health, and potential clinical applications
Identification of novel hyper- or hypomethylated CpG sites and genes associated with atherosclerotic plaque using an epigenome-wide association study
An evaluation of supervised methods for identifying differentially methylated regions in Illumina methylation arrays
Genome wide DNA methylation profiling identifies specific epigenetic features in high-risk cutaneous squamous cell carcinoma
Trimester-Specific Associations of Prenatal Lead Exposure With Infant Cord Blood DNA Methylation at Birth
Methylation of drug resistance-related genes in chemotherapy-sensitive Epstein-Barr virus-associated gastric cancer
Traditional Chinese Medicine Containing Arsenic Treated MDS Patients Effectively through Regulating Aberrant Hypomethylation
An empirically driven data reduction method on the human 450K methylation array to remove tissue specific non-variable CpGs
Young women with poor ovarian response exhibit epigenetic age acceleration based on evaluation of white blood cells using a DNA methylation-derived age prediction model
Genome-wide DNA methylation profiling in human breast tissue by illumina TruSeq methyl capture EPIC sequencing and infinium methylationEPIC beadchip microarray
Discordant DNA mismatch repair protein status between synchronous or metachronous gastrointestinal carcinomas: frequency, patterns, and molecular etiologies
CUE: CpG impUtation ensemble for DNA methylation levels across the human methylation450 (HM450) and EPIC (HM850) BeadChip platforms.
Adolescent sleep timing and dietary patterns in relation to DNA methylation of core circadian genes: a pilot study of Mexican youth
Maternal obesity is associated with a sex-specific epigenetic programming in human neonatal monocytes
Whole-Blood DNA Methylation Markers in Early Detection of Breast Cancer: A Systematic Literature Review
Identification and Validation of a 3-Gene Methylation Classifier for HPV-Based Cervical Screening on Self-Samples
Colorectal Carcinomas Containing Hypermethylated MLH1 Promoter and Wild-Type BRAF/KRAS Are Enriched for Targetable Kinase Fusions
Biomarkers of Exposure and Effect in the Lungs of Smokers, Nonsmokers, and Electronic Cigarette Users.
DNA Methylation-Derived Immune Cell Profiles, CpG Markers of Inflammation, and Pancreatic Cancer Risk.
Hyperactive CREB signaling pathway involved in the pathogenesis of polycystic ovarian syndrome revealed by patient-specific induced pluripotent stem cell modeling
Analysis and annotation of DNA methylation in two nonhuman primate species using the Infinium Human Methylation 450K and EPIC BeadChips.
Genome-wide methylation profiling in granulosa lutein cells of women with polycystic ovary syndrome (PCOS).
Twin study designs as a tool to identify new candidate genes for depression: A systematic review of DNA methylation studies
Colorectal cancer screening and diagnosis: omics-based technologies for development of a non-invasive blood-based method.
High-resolution analyses of human sperm dynamic methylome reveal thousands of novel age-related epigenetic alterations
MethCORR infers gene expression from DNA methylation and allows molecular analysis of ten common cancer types using fresh-frozen and formalin-fixed paraffin-embedded tumor samples.
Phase I trial of TRC102 (methoxyamine HCl) in combination with temozolomide in patients with relapsed solid tumors and lymphomas
Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA
CREs: Gene & Cell Therapy
Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.