Validation of a high-performance liquid chromatographic method for the enantiospecific quantitation of zopiclone in plasma

Biomedical Chromatography : BMC
Markus G Gebauer, Christopher P Alderman

Abstract

Zopiclone is a hypnosedative with clinical effects similar to benzodiazepines but thought to have less potential for rebound insomnia and withdrawal effects. Zopiclone is administered as a racemic mixture, and an enantiospecific method of analysis of zopiclone in plasma is desirable in the study of pharmacokinetic drug interactions. We report a modification of an HPLC method reported by Foster et al. using a closely related structural analogue of zopiclone as internal standard. Zopiclone was detected at 306 nm and linear calibration curves were constructed in the range of 1.0-250 ng/mL for each enantiomer. The % CV at 2.5 ng/mL was 12.0% for (-)-zopiclone and 14.3% for (+)-zopiclone, and the limit of quantification of each enantiomer was 2.5 ng/mL. At higher concentrations, the coefficient of variation was less than 10%. The nominal concentration of quality control samples was predicted with an accuracy within a range of +/-11.6%. The method was used in the analysis of plasma obtained from psychiatric patients. One sample obtained following a non-fatal overdose with zopiclone contained the metabolites (-)-N-oxide zopiclone and both enantiomers of desmethyl zopiclone. The metabolite enantiomers were resolved on the column with r...Continue Reading

References

Jan 1, 1983·Pharmacology·J GaillotJ F Dreyfus
Jan 1, 1982·International Pharmacopsychiatry·J GaillotJ F Dreyfus
Oct 1, 1994·British Journal of Clinical Pharmacology·K ArankoK T Olkkola
Aug 5, 1994·Journal of Chromatography. B, Biomedical Applications·R T FosterF M Pasutto
Jan 1, 1996·European Journal of Clinical Pharmacology·K M JalavaP J Neuvonen

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Citations

Feb 7, 2012·Bioanalysis·Milena Araújo Tonon, Pierina Sueli Bonato
Sep 21, 2010·Forensic Science International : Synergy·Gunnel H NilssonRobert Kronstrand
May 4, 2010·Forensic Science International : Synergy·Gunnel H NilssonJohan Ahlner
Jul 24, 2010·Journal of Pharmaceutical and Biomedical Analysis·Min MengPatrick K Bennett
May 28, 2011·Biomedical Chromatography : BMC·Kishore Kumar HothaV Venkateswarulu
Oct 31, 2014·Biomedical Chromatography : BMC·R Nageswara Rao, K Guru Prasad
Nov 7, 2012·Analytical and Bioanalytical Chemistry·Milena Araújo TononPierina Sueli Bonato
Jun 28, 2012·Electrophoresis·Milena Araújo Tonon, Pierina Sueli Bonato

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