Validation of a liquid chromatography tandem mass spectrometry assay for serum creatinine and comparison with enzymatic and Jaffe methods

Annals of Clinical Biochemistry
Laura J OwenBrian G Keevil

Abstract

Glomerular filtration rate (GFR) can be estimated using creatinine clearance or calculated formulae, methods which rely on creatinine quantification in serum. Creatinine is most frequently measured using the Jaffe method, but this is prone to interference by, for example, bilirubin, protein and ketones. Recent amendments to this assay have been made by manufacturers in an attempt to compensate for the protein interference. We developed a novel liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the analysis of creatinine in serum, using a strong cation exchange column to give a short run time of 1.5 min and minimal ion suppression. The method had a lower limit of quantification of 5 micromol/L and the intra- and inter-assay imprecision was <5% and <7%, respectively. Serum samples (n = 182) were subsequently analysed by enzymatic, compensated Jaffe and LC-MS/MS methods and results compared. The compensated Jaffe and enzymatic methods gave similar results to the LC-MS/MS method at concentrations below 150 micromol/L. At concentrations above this value, creatinine was underestimated by both the compensated Jaffe and enzymatic methods compared with the LC-MS/MS method. We have developed a novel, simple, sensitive L...Continue Reading

Citations

Feb 11, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Mario Fernández-FernándezJ Ignacio García Alonso
Jul 28, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Robert HarlanDanni Li Meany
Apr 1, 2008·Clinical Biochemistry·Michael Vogeser, Christoph Seger
Nov 21, 2007·Molecular Genetics and Metabolism·Christiane Auray-BlaisRégen Drouin
Aug 20, 2011·Pediatrics International : Official Journal of the Japan Pediatric Society·Benjamin D LakinRosaline Garr
Nov 30, 2018·Clinical Chemistry and Laboratory Medicine : CCLM·Roseri J A C Roelofsen-de BeerChristian Ramakers
Dec 1, 2006·Journal of Mass Spectrometry : JMS
Dec 26, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rajeshwar P MookerjeeRajiv Jalan
Nov 21, 2019·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Mukdawan SukhangKlintean Wunnapuk
Jan 30, 2007·Clinical Chemistry·Laura J Owen, Brian G Keevil
May 28, 2011·The Analyst·Chun-Che LinGwo-Bin Lee
Oct 22, 2008·Scandinavian Journal of Gastroenterology·Gustaf HerleniusStyrbjörn Friman
Jul 28, 2020·Annals of Clinical Biochemistry·David J MarshallBrian G Keevil
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Olga BegouDimitrios Tsikas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.