Validation of a method for measuring sperm quality and quantity in reproductive toxicity tests with pair-breeding male fathead minnows (Pimephales promelas)

ILAR Journal
David N HalaSusan Jobling

Abstract

The fathead minnow (Pimephales promelas) is an OECD-proposed test species routinely used in reproductive toxicity trials with suspected endocrine-disrupting compounds (EDCs). The basic fecundity, endocrinology, and histopathology of reproductively active male and female fathead minnows has been well characterized, but there are few studies of the utility of male sperm concentration and motility as endpoints for use in reproductive trials. The purpose of this study was to (1) characterize the baseline sperm concentration and motility of pair-breeding male fathead minnows over their spawning cycle and (2) determine whether a repeated and nondestructive sperm sampling protocol would influence the baseline fecundity of the fish. Pair-breeding male fathead minnows that underwent sampling for milt three times a week for 4 weeks exhibited no significant changes in milt volume, sperm concentration, or motility parameters up to 6 days after each spawning event. The repeated sperm sampling procedure did, however, cause a significant lowering of spawning frequencies, although this decline did not correlate with effects on fecundity as there were no significant changes in the mean total numbers of eggs laid, fertilization, and hatching suc...Continue Reading

Citations

Feb 13, 2001·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·K M JensenG T Ankley
May 17, 2011·Aquatic Toxicology·Patrick J MurackTerence P Barry
Nov 21, 2018·Zebrafish·Seyed-Mohammadreza SamaeeAlicia Estévez
May 2, 2018·Fish Physiology and Biochemistry·V GallegoJuan F Asturiano

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