Validation of a nanoliquid chromatography-tandem mass spectrometry method for the identification and the accurate quantification by isotopic dilution of glutathionylated and cysteinylated precursors of 3-mercaptohexan-1-ol and 4-mercapto-4-methylpentan-2-one in white grape juices

Journal of Chromatography. a
Aurélie RolandRémi Schneider

Abstract

A rapid nanoLC-MS/MS method was developed and validated for the simultaneous determination of glutathionylated and cysteinylated precursors of 3-mercapto-hexan-1-ol (3MH) and 4-methyl-4-mercaptopentan-2-one in grape juice using stable isotope dilution assay (SIDA). The analytes were extracted from must using a cation exchange resin and purified on C18 cartridges. They were chromatographically separated on a reverse phase column and finally analyzed by tandem mass spectrometry in selected reaction monitoring mode (SRM) using deuterated analogues as standards except for glutathionylated conjugate of 4MMP which was analyzed by external calibration. The method was validated according to the International Conference on Harmonization recommendations by determining linearity, accuracy, precision, recovery, matrix effect, repeatability, intermediate reproducibility, LODs and LOQs. Calibration for each precursor was determined by performing Lack-of-Fit test and the best fitting for 3MH precursors was a quadratic model whereas a linear model was better adapted for 4MMP precursors. All calibration curves showed quite satisfactory correlation coefficients (R(2)>0.995 for SIDA quantification and R(2)>0.985 for external calibration). Quantif...Continue Reading

References

Mar 14, 2007·Journal of Chromatography. a·Javier Hernández-BorgesSalvatore Fanali
Mar 27, 2009·Journal of Chromatography. a·Katia BuonaseraLuigi Mondello

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Citations

Jul 26, 2011·Chemical Reviews·Aurélie RolandFlorine Cavelier
Sep 5, 2013·Food Chemistry·Peggy RigouAlain Razungles
Jul 24, 2014·Food Chemistry·Roberto LarcherGiorgio Nicolini
Feb 23, 2020·Journal of the Science of Food and Agriculture·Giorgio NicoliniRoberto Larcher
Aug 5, 2019·Applied Microbiology and Biotechnology·Javier RuizSantiago Benito
Oct 22, 2020·Toxicology Mechanisms and Methods·Lailah Cristina de Carvalho AbrãoIsarita Martins
Mar 5, 2010·Journal of Agricultural and Food Chemistry·Aurélie RolandRémi Schneider
Sep 10, 2010·Journal of Agricultural and Food Chemistry·Aurélie RolandFlorine Cavelier

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