Validation of a novel biomarker for acute axonal injury in experimental autoimmune encephalomyelitis

Journal of Neuroscience Research
Melissa GresleHelmut Butzkueven

Abstract

In multiple sclerosis, inflammatory axonal injury is a key pathological mechanism responsible for the development of progressive neurological dysfunction. The injured axon represents a therapeutic target in this disease; however, therapeutic trials of neuroprotective candidates will initially require preclinical testing in an animal model of inflammatory axonal injury and subsequently the development of a reliable paraclinical measure of axonal degeneration in humans. In the present study, we demonstrate the validity of serum phosphorylated neurofilament H (pNF-H) as a marker of axonal injury in murine experimental autoimmune encephalomyelitis (EAE). At the time of maximum disease severity (EAE day 22), the average serum pNF-H level reached 5.7 ng/ml, correlating significantly with the EAE paraplegia score (r = 0.75, P < 0.001). On average, 40% of axons in the spinal cord were lost in EAE, and serum pNF-H levels were highly correlated with axon loss (r = 0.8, P < 0.001). Axonal injury was a severe and acute event, insofar as serum pNF-H levels were not significantly elevated at early (EAE day 12) or late (EAE days 35 and 50) disease time points. Our results demonstrate that acute inflammatory axonal injury is a pathological fea...Continue Reading

References

Jan 1, 1994·Annual Review of Biochemistry·E Fuchs, K Weber
Jan 29, 1998·The New England Journal of Medicine·B D TrappL Bö
Jan 29, 1998·The New England Journal of Medicine·S G Waxman
Apr 4, 1998·Journal of Neurology, Neurosurgery, and Psychiatry·J N LyckeL E Rosengren
Jan 23, 1999·Annals of Neurology·Z SahenkJ R Mendell
Sep 28, 1999·Current Opinion in Neurology·B D TrappR Rudick
May 29, 2000·Brain : a Journal of Neurology·A BitschW Brück
Jun 4, 2002·Nature Medicine·Helmut ButzkuevenTrevor J Kilpatrick
May 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Matthew J CranerStephen G Waxman
Jan 18, 2005·Journal of Neurology, Neurosurgery, and Psychiatry·A PetzoldG Giovannoni
May 18, 2005·Journal of the Neurological Sciences·Axel Petzold
Aug 19, 2005·Journal of Medicinal Chemistry·Laëtitia BouératChristina Andersson
Sep 24, 2005·Biochemical and Biophysical Research Communications·Gerry ShawDena R Howland
Oct 1, 2005·Multiple Sclerosis : Clinical and Laboratory Research·E T LimG Giovannoni
Apr 1, 2006·Trends in Molecular Medicine·Stephen G Waxman
Dec 5, 2006·Journal of Immunological Methods·Axel Petzold, Gerry Shaw
May 12, 2007·Journal of the Neurological Sciences·P G Bannerman, A Hahn

❮ Previous
Next ❯

Citations

Sep 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·David C WraithDavid Wynick
Dec 15, 2010·Journal of Veterinary Internal Medicine·N C RinggerG Shaw
Nov 19, 2011·Multiple Sclerosis International·Melissa M GresleGerry Shaw
Jun 1, 2013·Investigative Ophthalmology & Visual Science·Venu TallaJohn Guy
Nov 21, 2013·Acta Neuropathologica Communications·Vilija G JokubaitisHelmut Butzkueven
Oct 21, 2014·The Journal of Clinical Investigation·Anna JonasMelissa Gresle
Jul 23, 2015·Proteomics. Clinical Applications·Antoine MénoretAnthony T Vella
May 8, 2010·Journal of Neuropathology and Experimental Neurology·Steven PetratosClaude C A Bernard
Dec 17, 2015·Journal of Neuroinflammation·L SchreweA Chan
Nov 19, 2011·Multiple Sclerosis International·Irena Dujmovic
Aug 1, 2015·Autoimmunity Reviews·Antonella D'AmbrosioPaola Margutti
Apr 2, 2014·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·J SellnerA Petzold
Feb 11, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Kina HöglundErik Portelius
Oct 3, 2013·Molecular Immunology·Ning MaRenxi Wang
Feb 22, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Therese RosenlingRainer Bischoff
May 9, 2014·Journal of the Neurological Sciences·Ana Rodríguez-RodríguezFrancisco Murillo-Cabezas
Mar 19, 2014·Journal of Neurology, Neurosurgery, and Psychiatry·M M GresleH Butzkueven
Apr 19, 2016·Multiple Sclerosis Journal - Experimental, Translational and Clinical·Melissa M GresleSha Mi
Oct 25, 2013·Expert Review of Molecular Diagnostics·Kina Höglund, Hugh Salter
Sep 16, 2016·Journal of Neuroinflammation·Henrik HasseldamFlemming Fryd Johansen
Mar 29, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ning MaRenxi Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.