Validation of a pan-orthopox real-time PCR assay for the detection and quantification of viral genomes from nonhuman primate blood

Virology Journal
Eric M MuckerJohn Huggins

Abstract

In 1980, smallpox disease was eradicated from nature and Variola virus, the etiological agent of smallpox, was confined to two laboratories, one located in Russia (Moscow) later moved to VECTOR (Novosibirsk, Siberia) and one in the United States (CDC Atlanta). Vaccinations among the general public ceased shortly after the successful eradication campaign, resulting in an increasingly immunologically susceptible population. Because of the possibility of intentional reintroduction of Variola virus and the emergence of other pathogenic poxviruses, there is a great need for the development of medical countermeasures to treat poxvirus disease. It is highly likely that the U.S. FDA "animal rule" will be necessary for regulatory approval of these interventions. Therefore, relevant animal models and the associated supporting assays will require development to stand up to regulatory scrutiny. An optimized real time PCR assay for the detection of orthopoxviruses has been developed by researchers at the United States Army Research Institute of Infectious Diseases (USAMRIID). To support animal studies that will be used to support approval of medical countermeasures by the U.S. FDA, the assay was designed to quantitate poxvirus genomic DNA i...Continue Reading

References

Jun 23, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·David A KuleshGeorge V Ludwig
Apr 8, 2009·Antimicrobial Agents and Chemotherapy·John HugginsDennis Hruby
Mar 11, 2011·Journal of Virology·Arthur J GoffLisa Hensley
Oct 9, 2013·Antimicrobial Agents and Chemotherapy·Eric M MuckerDennis E Hruby

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Methods Mentioned

BETA
electrophoresis
PCR
Assay

Software Mentioned

Excel
Aliquot Gap Analysis
LightCycler®

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