Abstract
Tumor biomarker studies are integral to oncology clinical trials but may yield artifactual results owing to variation in sample procurement and processing. Ethanol, 70% vol/vol, was validated as a sample transport medium using markers of the PI3K/Akt/mTOR pathway. BT474 tumor xenografts were excised and slices were immediately placed into formaldehyde and fixed for 24 hours. Fixed tissue slices were immediately processed into paraffin or transferred to 70% vol/vol ethanol and stored at room temperature for 1, 2, and 4 weeks before further processing. Freshly cut tissue sections were evaluated for pAKT(S473), HER2, pHER-2(Y1248), pS6(S235/236), and pS6(S240/244), Ki-67, and HER2 by fluorescence in situ hybridization and stained with H&E and Masson trichrome. No significant changes were observed when comparing samples stored in 70% ethanol for up to 4 weeks with immediately processed tissue. Ethanol, 70% vol/vol, provides a safe storage medium for formaldehyde-fixed tumor tissue, facilitating sample transport during multicenter clinical trials.
References
May 1, 1995·Journal of Clinical Pathology·V Jensen, M Ladekarl
Feb 1, 1994·Journal of Clinical Pathology·K Y ChiuF C Ho
May 1, 1994·The Journal of Pathology·F Penault-LlorcaJ Jacquemier
Jan 1, 1997·Analytical Cellular Pathology : the Journal of the European Society for Analytical Cellular Pathology·D G VinceR R Tubbs
Jul 15, 2000·The American Journal of Surgical Pathology·M WernerH Battifora
Dec 18, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Sathiyamoorthy SelvarajanPuay-Hoon Tan
Oct 28, 2003·The American Journal of Pathology·James W Mandell
Jun 23, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Derek AtkinsStephan Störkel
Jun 24, 2004·BioTechniques·Annika SpruesselKerstin A David
Oct 28, 2004·The Journal of Molecular Diagnostics : JMD·Mark A PerlmutterRodrigo F Chuaqui
Jun 17, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Amanda F BakerGarth Powis
Jul 29, 2005·Histochemistry and Cell Biology·Dorthe von SmolinskiAchim D Gruber
May 1, 2007·Brain Pathology·Isidre FerrerHans Kretzschmar
Dec 7, 2007·PloS One·Silke von AhlfenMartin Schlumpberger
Mar 29, 2008·Methods in Molecular Biology·Dean Troyer
Jul 24, 2008·Histochemistry and Cell Biology·James W Mandell
Nov 13, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Janine A BurnsZhi-Qiang Zhang
Apr 7, 2009·Methods in Molecular Biology·Yelena Golubeva, Keith Rogers
Apr 22, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·José BaselgaHope S Rugo
Jun 11, 2010·Nature Reviews. Cancer·Timothy A YapJohann S de Bono
Sep 8, 2010·The New England Journal of Medicine·Keith T FlahertyPaul B Chapman