PMID: 2100608Jan 1, 1990Paper

Validation of a radioimmunoassay for the determination of human plasma concentrations of lamotrigine

Journal of Pharmaceutical and Biomedical Analysis
R A BiddlecombeS C Jeal

Abstract

A precise and sensitive radioimmunoassay to determine human plasma lamotrigine (430C78), Lamictal) is described. The method is a direct double antibody procedure employing a rabbit polyclonal first antibody raised to a BSA conjugate of lamotrigine, and an iodinated tyrosine methyl ester of lamotrigine as the tracer. Both reagents are added simultaneously to samples containing lamotrigine prior to an overnight incubation at 4 degrees C. The method has a sensitivity of 20 ng ml-1, when plasma samples are initially diluted 1:20 with phosphate buffer and sample volumes of 20 microliters are used. The intra-assay precision at 40, 80 and 160 ng ml-1 was 6.2, 2.1 and 4.8%, respectively, and the inter-assay precision at 500, 2000, 5000 and 10,000 ng ml-1 was 4.6, 5.7, 4.6 and 5.9%, respectively. The method was specific and showed reasonable correlation with an HPLC method [1].

References

Jun 1, 1969·The Journal of Clinical Endocrinology and Metabolism·G E Abraham

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Citations

Apr 1, 1996·Journal of Pharmaceutical and Biomedical Analysis·E ForssbladO Beck
Jan 19, 2007·Biomedical Chromatography : BMC·Elizabeth Greiner-SosankoMatthew D Krasowski
Jul 20, 2010·Pharmaceuticals·Matthew D Krasowski
Jun 11, 2011·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Juseop KangMin-Young Jun
Mar 7, 2021·Critical Reviews in Analytical Chemistry·Sajeeda SRuchi Verma
Mar 12, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Daniel F Chollet

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