Validation of a self-excising marker in the human pathogen Aspergillus fumigatus by employing the beta-rec/six site-specific recombination system.

Applied and Environmental Microbiology
Thomas HartmannSven Krappmann

Abstract

Recyclable markers based on site-specific recombination allow repetitive gene targeting in filamentous fungi. Here we describe for the first time functionality of the bacterial recombination system employing beta serine recombinase acting on six recognition sequences (beta-rec/six) in a fungal host, the human pathogen Aspergillus fumigatus, and its use in establishing a self-excising resistance marker cassette for serial gene replacement.

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Citations

Sep 12, 2012·Antimicrobial Agents and Chemotherapy·Cristina Jiménez-OrtigosaJean-Paul Latgé
Apr 17, 2012·Applied and Environmental Microbiology·Osamu MizutaniHaruyuki Iefuji
Jan 11, 2014·Applied Microbiology and Biotechnology·Sven Krappmann
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Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

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